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Pancreatic Cancer pp 143–148Cite as

Osteoclast Differentiation Assay

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1882))

Abstract

Osteoclasts are highly specialized multinucleated cells derived from the monocyte/macrophage hematopoietic lineage that are uniquely capable of adhering to bone matrix and resorbing bone. The tartrate-resistant acid phosphatase (TRAP) assay is the most common method to detect osteoclasts population in vitro. Here we described a general protocol of inducing osteoclast differentiation from the murine macrophage cell line, RAW264.7, and identification of osteoclasts with the classical TRAP assay.

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Author information

Authors and Affiliations

  1. Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Jingxuan Yang & Min Li

  2. Department of Surgery, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Jingxuan Yang & Min Li

  3. Department of Nanomedicine and Biomedical Engineering, The University of Texas Medical School at Houston, Houston, TX, USA

    Xiaohong Bi

Authors
  1. Jingxuan Yang
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  2. Xiaohong Bi
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  3. Min Li
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Corresponding authors

Correspondence to Xiaohong Bi or Min Li .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Gloria H. Su

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Yang, J., Bi, X., Li, M. (2019). Osteoclast Differentiation Assay. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 1882. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8879-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8879-2_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8878-5

  • Online ISBN: 978-1-4939-8879-2

  • eBook Packages: Springer Protocols

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