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Poplar Transformation

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Book cover Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

First publications of successful Agrobacterium-mediated transformation of tobacco were published more than 30 years ago. Protocols for Agrobacterium-based transformation as well as biolistic bombardment and PEG transformation of protoplasts are available for more than 150 plant species from various plant families. Also for many Populus species and hybrids, adapted transformation protocols have been published. The standard protocol for Agrobacterium-mediated transformation of different Populus genotypes is the leaf-disc method. Here, we first describe the transfer of genes into poplar by using the Agrobacterium-based leaf disc methods. In addition, alternative basic transformation methods, namely, biolistic bombardment and PEG transformation of protoplasts, are also described. Further, we present improved poplar transformation protocols by simplifying the transformation procedure and optimizing tissue preparation and plant regeneration.

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Acknowledgments

We would like to thank all technical assistants of the genome research group for valuable input, namely, Susanne Jelkmann, Denise Lehnhardt, Jennifer Lüneburg, Anke Schellhorn, and Doris Ebbinghaus.

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Authors and Affiliations

  1. Thuenen Institute of Forest Genetics, Grosshansdorf, Germany

    Tobias Bruegmann, Olaf Polak, Khira Deecke & Matthias Fladung

  2. CGS Crop Genetic Systems, Hamburg, Germany

    Julia Nietsch

Authors
  1. Tobias Bruegmann
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  2. Olaf Polak
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  3. Khira Deecke
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  4. Julia Nietsch
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  5. Matthias Fladung
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Corresponding author

Correspondence to Matthias Fladung .

Editor information

Editors and Affiliations

  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Michelle Smith

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Bruegmann, T., Polak, O., Deecke, K., Nietsch, J., Fladung, M. (2019). Poplar Transformation. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

  • eBook Packages: Springer Protocols

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