Abstract
High-resolution fluorescence microscopy is increasingly contributing to our understanding of molecular processes. By utilizing single-molecule intensity information, imaging experiments can be rendered quantitative, yielding insights into the stoichiometry and kinetics of the components of a molecular assembly. Here, we describe the experimental and analytical steps needed to study the assembly of clathrin-coated vesicles with single-molecule resolution, using total internal reflection fluorescence microscopy. Many components of the protocol are broadly applicable to the characterization of other molecular processes.
The original version of this chapter was revised. A correction to this chapter can be found at https://doi.org/10.1007/978-1-4939-8719-1_19
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Change history
24 October 2018
This book was inadvertently published with the incorrect title as Clathrin-Mediated Endoytosis: Methods and Protocols. This has now been corrected throughout the book to Clathrin-Mediated Endocytosis: Methods and Protocols.
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Acknowledgments
Dr. Cocucci was supported by The Ohio State University Comprehensive Cancer Center (P30-CA016058) and an intramural fund (IRP46050-502339) granted to Dr. Cocucci.
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Aguet, F., Cocucci, E. (2018). Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution. In: Swan, L. (eds) Clathrin-Mediated Endocytosis. Methods in Molecular Biology, vol 1847. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8719-1_15
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DOI: https://doi.org/10.1007/978-1-4939-8719-1_15
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