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Lymphangiogenesis pp 301–308Cite as

Isolation and Characterization of Mouse Organ-Specific Endothelial Transcriptomes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1846))

Abstract

Endothelial cells play an essential role in homeostasis and regeneration and their transcriptional profile has been found to be tissue specific. Here we describe the isolation of organ specific mouse endothelial cells using either magnetic beads or fluorescence-activated cell sorting. This efficient protocol should allow for the transcriptional characterization of organotypic endothelial cells.

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References

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Author information

Authors and Affiliations

  1. Department of Regenerative Medicine, Weill Cornell Medical College, New York, NY, USA

    Shahin Rafii & Brisa Palikuqi

Authors
  1. Shahin Rafii
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  2. Brisa Palikuqi
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Corresponding author

Correspondence to Brisa Palikuqi .

Editor information

Editors and Affiliations

  1. Center for Vascular & Developmental Biology, Feinberg Cardiovascular Research Institute, Northwestern University, Chicago, IL, USA

    Guillermo Oliver

  2. Department of Medicine and Cardiovascular Institute, University of Pennsylvania, Philadelphia, PA, USA

    Mark L. Kahn

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Rafii, S., Palikuqi, B. (2018). Isolation and Characterization of Mouse Organ-Specific Endothelial Transcriptomes. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8712-2_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8711-5

  • Online ISBN: 978-1-4939-8712-2

  • eBook Packages: Springer Protocols

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