Abstract
The protocol presented here allows the isolation, purification, nucleic acid extraction, and amplification of DNA/RNA from viruses present in human sera samples. The method allows the random amplification of the viral genomes present by using a Sequence-Independent, Single-Primer Amplification (SISPA) approach enabling the study of both DNA/RNA viruses. An amplification step is needed, as the concentration of viral DNA/RNA in serum samples is low for direct library preparation. The application of the described protocol guarantees enough randomly amplified double-strand DNA for further library preparation using Nextera XT kit from Illumina.
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Fernandez-Cassi, X., Rusiñol, M., Martínez-Puchol, S. (2018). Viral Concentration and Amplification from Human Serum Samples Prior to Application of Next-Generation Sequencing Analysis. In: Moya, A., Pérez Brocal, V. (eds) The Human Virome. Methods in Molecular Biology, vol 1838. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8682-8_13
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DOI: https://doi.org/10.1007/978-1-4939-8682-8_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8682-8
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