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Serpins in Venous Thrombosis and Venous Thrombus Resolution

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Serpins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1826))

Abstract

Several serpins function as potent inhibitors of thrombolytic serine proteases. Venous thrombosis is a common and debilitating condition whose incidence is on the rise. Studies using genetically modified mice and inhibitors have shown that the plasminogen activator inhibitors (PAI), PAI-1 and PAI-2, are primary regulators of plasminogen activation and contribute to regulating the resolution of experimental venous thrombi, via inflammatory mechanisms, vascular remodeling, and inhibition of fibrinolysis. Therapies to accelerate venous thrombus resolution would be beneficial, since delayed or incomplete clot resolution frequently leads to postthrombotic syndrome, a long-term complication associated with debilitating limb swelling, pain, and recurrent skin ulceration. Here we describe a useful and reproducible mouse model for the study of venous thrombus resolution involving ligation of the inferior vena cava and elucidation of the molecular and cellular determinants of venous thrombus formation and resolution.

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Acknowledgments

This work was supported by award number 1I01BX001921 (TMA) from the Biomedical Laboratory Research & Development Service of the Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, and by the National Institutes of Health T32 HL007698 (TAJ).

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Authors and Affiliations

  1. Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, USA

    Subhradip Mukhopadhyay, Tierra A. Johnson, Rajabrata Sarkar & Toni M. Antalis

  2. Departments of Surgery and Physiology, University of Maryland School of Medicine, Baltimore, MD, USA

    Subhradip Mukhopadhyay, Rajabrata Sarkar & Toni M. Antalis

  3. Veterans Affairs Maryland Health Care System, Baltimore, MD, USA

    Toni M. Antalis

Authors
  1. Subhradip Mukhopadhyay
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  2. Tierra A. Johnson
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  3. Rajabrata Sarkar
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  4. Toni M. Antalis
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Corresponding author

Correspondence to Toni M. Antalis .

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Editors and Affiliations

  1. Biodesign Center for Personalized Diagnostics and Center for Immunotherapy Vaccines and Virotherapy, Arizona State University, Tempe, AZ, USA

    Alexandra Lucas

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Mukhopadhyay, S., Johnson, T.A., Sarkar, R., Antalis, T.M. (2018). Serpins in Venous Thrombosis and Venous Thrombus Resolution. In: Lucas, A. (eds) Serpins. Methods in Molecular Biology, vol 1826. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8645-3_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8645-3_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8644-6

  • Online ISBN: 978-1-4939-8645-3

  • eBook Packages: Springer Protocols

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