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Serpins pp 143–155Cite as

In Vivo Analysis of Alpha-1-Antitrypsin Functions in Autoimmune Disease Models

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1826))

Abstract

Alpha-1-antitrypsin (AAT) is a circulating protein, a serpin, with multiple protective functions. Beside the well-known proteinase inhibitory function, which protects the lungs from chronic obstructive pulmonary disease (COPD), many studies have shown that AAT inhibits pro-inflammatory cytokine gene expression and functions. These anti-inflammatory and immune-regulatory properties have led to studies testing the therapeutic effect of AAT in autoimmune disease models. Initially, a study using recombinant adeno-associated viral (rAAV) vector showed that AAT gene therapy prevented type 1 diabetes (T1D) development in a nonobese diabetic (NOD) mouse model. Consequently, several studies confirmed that AAT therapy prevented and reversed T1D. AAT therapy has also been tested and has demonstrated protective effects in a collagen-induced arthritis model and a systemic lupus erythematosus (SLE) mouse model. This chapter describes methods that evaluate AAT functions in autoimmune mouse models.

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Authors and Affiliations

  1. Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, FL, USA

    Sihong Song, Yuanqing Lu & Ahmed S. Elshikha

Authors
  1. Sihong Song
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  2. Yuanqing Lu
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  3. Ahmed S. Elshikha
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Correspondence to Sihong Song .

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Editors and Affiliations

  1. Biodesign Center for Personalized Diagnostics and Center for Immunotherapy Vaccines and Virotherapy, Arizona State University, Tempe, AZ, USA

    Alexandra Lucas

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Song, S., Lu, Y., Elshikha, A.S. (2018). In Vivo Analysis of Alpha-1-Antitrypsin Functions in Autoimmune Disease Models. In: Lucas, A. (eds) Serpins. Methods in Molecular Biology, vol 1826. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8645-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8645-3_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8644-6

  • Online ISBN: 978-1-4939-8645-3

  • eBook Packages: Springer Protocols

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