Abstract
In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modeling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about troubleshooting and optimizing such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced pluripotent stem cells into cardiomyocytes.
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Acknowledgments
We are grateful to Po-Lin So and Bruce Conklin (Gladstone Institutes) for providing a modified version of the Lian et al. protocol for cardiomyocyte differentiation from human ES cells. We thank Kate Watt, and Yvonne Turnbull (University of Aberdeen), for lab management and technical support, Rory J Bonner (University of Aberdeen) for providing images, the British Heart Foundation (PG/12/75/29851) for research funding, and the Institute of Medical Sciences (University of Aberdeen) for PhD studentship funding.
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Mazzotta, S., Lynch, A.T., Hoppler, S. (2018). Cardiomyocyte Differentiation from Human Embryonic Stem Cells. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_5
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DOI: https://doi.org/10.1007/978-1-4939-8597-5_5
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