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Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches

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Lung Innate Immunity and Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1809))

Abstract

Transgenic mice have emerged as a central tool in the study of lung myeloid cells during homeostasis and disease. The use of Cre/Lox site-specific recombination allows for conditional deletion of a gene of interest in a spatially controlled manner. The basic Cre/Lox system can be further refined to include an inducible trigger, enabling conditional deletion of a gene of interest in a spatially and temporally controlled manner. Here we provide an overview of commercially available conditional and inducible conditional mouse strains that target lung myeloid cells and describe the appropriate breeding schemes and controls for transgenic animal systems that can be used to modulate myeloid cell function.

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Authors and Affiliations

  1. Department of Medicine, National Jewish Health, Denver, CO, USA

    Alexandra L. McCubbrey

  2. Division of Critical Care Medicine and Pulmonary Sciences, Department of Medicine, University of Colorado, Denver, CO, USA

    Alexandra L. McCubbrey

  3. Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, National Jewish Health, Denver, CO, USA

    William J. Janssen

  4. Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Denver, Aurora, CO, USA

    William J. Janssen

Authors
  1. Alexandra L. McCubbrey
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  2. William J. Janssen
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Correspondence to Alexandra L. McCubbrey .

Editor information

Editors and Affiliations

  1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, Colorado, USA

    Scott Alper

  2. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, Colorado, USA

    William J. Janssen

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McCubbrey, A.L., Janssen, W.J. (2018). Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8570-8_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8569-2

  • Online ISBN: 978-1-4939-8570-8

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