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Methods to Study the Role of Progranulin in the Tumor Microenvironment

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Progranulin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1806))

Abstract

Accurate measurement of progranulin (PGRN) in the circulation and in the tumor microenvironment is essential for understanding its role in cancer progression and metastasis. This chapter describes a number of approaches to measure the transcription level of the GRN gene and to detect and analyze PGRN expression in cancer cells and in the local environment of the tumor, in mouse and human samples. These validated protocols are utilized to investigate the functional role of PGRN in cancer. Finally, we discuss strategies to investigate the functions of PGRN in tumors using genetically modified mouse models and gene silencing techniques.

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Author information

Authors and Affiliations

  1. The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel

    Moshe Elkabets

  2. Human Oncology and Pathogenesis Program (HOPP), Memorial Sloan Kettering Cancer Center, New York, NY, USA

    Samuel Brook

Authors
  1. Moshe Elkabets
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  2. Samuel Brook
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Corresponding author

Correspondence to Moshe Elkabets .

Editor information

Editors and Affiliations

  1. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Andrew Bateman

  2. Department of Medicine, McGill University MUHC, Montreal, Québec, Canada

    Hugh P.J. Bennett

  3. Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China

    Siu Tim Cheung

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Elkabets, M., Brook, S. (2018). Methods to Study the Role of Progranulin in the Tumor Microenvironment. In: Bateman, A., Bennett, H., Cheung, S. (eds) Progranulin. Methods in Molecular Biology, vol 1806. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8559-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-8559-3_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8557-9

  • Online ISBN: 978-1-4939-8559-3

  • eBook Packages: Springer Protocols

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