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Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples

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Thyroid Hormone Nuclear Receptor

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1801))

Abstract

Thyroid hormone is a principal regulator of essential processes in vertebrate physiology and homeostasis. Synthetic derivatives of thyroid hormone, known as thyromimetics, display desirable therapeutic properties. Thoroughly understanding how thyromimetics distribute throughout the body is crucial for their development and this requires appropriate bioanalytical techniques to quantify drug levels in different tissues. Here, we describe a detailed protocol for the quantification of the thyromimetic sobetirome using liquid chromatography tandem-mass spectrometry (LC-MS/MS).

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Authors and Affiliations

  1. Program in Chemical Biology, Department of Physiology & Pharmacology, Oregon Health & Science University, Portland, OR, USA

    Jordan Devereaux, Skylar J. Ferrara & Thomas S. Scanlan

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  1. Jordan Devereaux
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  2. Skylar J. Ferrara
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  3. Thomas S. Scanlan
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Correspondence to Thomas S. Scanlan .

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Editors and Affiliations

  1. Centre Léon Bérard, Cancer Research Center of Lyon, Lyon, France

    Michelina Plateroti

  2. École normale supérieure de Lyon, Lyon, France

    Jacques Samarut

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Devereaux, J., Ferrara, S.J., Scanlan, T.S. (2018). Quantification of Thyromimetic Sobetirome Concentration in Biological Tissue Samples. In: Plateroti, M., Samarut, J. (eds) Thyroid Hormone Nuclear Receptor. Methods in Molecular Biology, vol 1801. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7902-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7902-8_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7901-1

  • Online ISBN: 978-1-4939-7902-8

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