Abstract
Large animal models offer novel opportunities in exploring safety, biology, and efficacy of novel therapeutic approaches for Huntington’s disease (HD). Challenges in the development of, for example, gene therapy, such as delivery, distribution, and persistence of virus vectors or oligo sense nucleotides, can be explored in large brains and organisms approaching human size. We here introduce the transgenic Libechov minipig as a large animal model of HD. Methods developed to assess motor, cognitive, and behavioral features expected to manifest in an HD model are described. We also outline established protocols for magnetic resonance imaging (MRI) including magnetic resonance spectroscopy (MRS) for minipigs. The successful conduct of long-term follow-up studies over several years with repeated behavioral testing and imaging is reported. We discuss the advantages and limitations of using this model with regard to translational reliability, homology to humans and with respect to feasibility, breeding, housing, handling, and finally ethical considerations. It is concluded that minipigs can fulfill an important role in preclinical development to bridge the gap between rodents and nonhuman primate research in the translation to humans.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Morton AJ, Howland DS (2013) Large genetic animal models of Huntington’s disease. J Huntingtons Dis 2:3–19
Miniarikova J, Zanella I, Huseinovic A et al (2016) Design, characterization, and lead selection of therapeutic mirnas targeting huntingtin for development of gene therapy for Huntington’s disease. Mol Ther Nucleic Acids 5:e297
Baxa M, Hruska-Plochan M, Juhas S et al (2013) A transgenic minipig model of Huntington’s disease. J Huntingtons Dis 2:47–68
Aylward EH, Sparks BF, Field KM et al (2004) Onset and rate of striatal atrophy in preclinical Huntington disease. Neurology 63:66–72
Tabrizi SJ, Langbehn DR, Leavitt BR et al (2009) Biological and clinical manifestations of Huntington’s disease in the longitudinal TRACK-HD study: cross-sectional analysis of baseline data. Lancet Neurol 8:791–801
Bechtel N, Scahill RI, Rosas HD et al (2010) Tapping linked to function and structure in premanifest and symptomatic Huntington disease. Neurology 75:2150–2160
Tabrizi SJ, Scahill RI, Owen G et al (2013) Predictors of phenotypic progression and disease onset in premanifest and early-stage Huntington’s disease in the TRACK-HD study: analysis of 36-month observational data. Lancet Neurol 12:637–649
Tabrizi SJ, Reilmann R, Roos RA et al (2012) Potential endpoints for clinical trials in premanifest and early Huntington’s disease in the TRACK-HD study: analysis of 24 month observational data. Lancet Neurol 11:42–53
Paulsen JS, Langbehn DR, Stout JC et al (2008) Detection of Huntington’s disease decades before diagnosis: the Predict-HD study. J Neurol Neurosurg Psychiatry 79:874–880
Paulsen JS, Long JD, Ross CA et al (2014) Prediction of manifest Huntington’s disease with clinical and imaging measures: a prospective observational study. Lancet Neurol 13:1193–1201
Sturrock A, Laule C, Wyper K et al (2015) A longitudinal study of magnetic resonance spectroscopy Huntington’s disease biomarkers. Mov Disord 30:393–401
Sturrock A, Laule C, Decolongon J et al (2010) Magnetic resonance spectroscopy biomarkers in premanifest and early Huntington disease. Neurology 75:1702–1710
Sampaio C, Borowsky B, Reilmann R (2014) Clinical trials in Huntington’s disease: interventions in early clinical development and newer methodological approaches. Mov Disord 29:1419–1428
Reilmann R, Rouzade-Dominguez ML, Saft C et al (2015) A randomized, placebo-controlled trial of AFQ056 for the treatment of chorea in Huntington’s disease. Mov Disord 30:427–431
Reilmann R, McGarry A, Landwehrmeyer GB et al (2017) Efficacy, safety, and tolerability of pridopidine in Huntington’s disease (HD): results from the phase II dose-ranging study, Pride-HD. Mov Disord 32(Suppl S2):323–324
Reilmann R, Schubert R (2017) Motor outcome measures in Huntington disease clinical trials. In: Feigin A, Anderson KE (eds) Huntington disease. Elsevier B.V., New York
Huntington Study Group (1996) Unified Huntington’s Disease Rating Scale: reliability and consistency. Mov Disord 11:136–142
Howland DS, Munoz-Sanjuan I (2014) Mind the gap: models in multiple species needed for therapeutic development in Huntington’s disease. Mov Disord 29:1397–1403
Rosser A, Svendsen CN (2014) Stem cells for cell replacement therapy: a therapeutic strategy for HD? Mov Disord 29:1446–1454
Schramke S, Schuldenzucker V, Schubert R et al (2016) Behavioral phenotyping of minipigs transgenic for the Huntington gene. J Neurosci Methods 265:34–45
Rao AK, Muratori L, Louis ED et al (2008) Spectrum of gait impairments in presymptomatic and symptomatic Huntington’s disease. Mov Disord 23:1100–1107
Grimbergen YA, Knol MJ, Bloem BR et al (2008) Falls and gait disturbances in Huntington’s disease. Mov Disord 23:970–976
Reilmann R, Rumpf S, Beckmann H et al (2012) Huntington’s disease: objective assessment of posture—a link between motor and functional deficits. Mov Disord 27:555–559
Rao AK, Mazzoni P, Wasserman P, Marder K (2011) Longitudinal change in gait and motor function in pre-manifest Huntington’s disease. PLoS Curr 3:RRN1268
Scahill RI, Hobbs NZ, Say MJ et al (2013) Clinical impairment in premanifest and early Huntington’s disease is associated with regionally specific atrophy. Hum Brain Mapp 34:519–529
Reilmann R, Bohlen S, Klopstock T et al (2010) Tongue force analysis assesses motor phenotype in premanifest and symptomatic Huntington’s disease. Mov Disord 25:2195–2202
Skodda S, Schlegel U, Hoffmann R, Saft C (2014) Impaired motor speech performance in Huntington’s disease. J Neural Transm 121:399–407
Heemskerk AW, Roos RA (2011) Dysphagia in Huntington’s disease: a review. Dysphagia 26:62–66
Walker FO (2007) Huntington’s disease. Lancet 369:218–228
Dumas EM, van den Bogaard SJ, Middelkoop HA, Roos RA (2013) A review of cognition in Huntington’s disease. Front Biosci (Schol Ed) 5:1–18
Ross CA, Pantelyat A, Kogan J, Brandt J (2014) Determinants of functional disability in Huntington’s disease: role of cognitive and motor dysfunction. Mov Disord 29:1351–1358
Stout JC, Jones R, Labuschagne I et al (2012) Evaluation of longitudinal 12 and 24 month cognitive outcomes in premanifest and early Huntington’s disease. J Neurol Neurosurg Psychiatry 83:687–694
Paulsen JS, Long JD, Johnson HJ et al (2014) Clinical and biomarker changes in premanifest Huntington disease show trial feasibility: a decade of the PREDICT-HD study. Front Aging Neurosci 6:78
Lione LA, Carter RJ, Hunt MJ et al (1999) Selective discrimination learning impairments in mice expressing the human Huntington’s disease mutation. J Neurosci 19:10428–10437
Craufurd D, Thompson JC, Snowden JS (2001) Behavioral changes in Huntington disease. Neuropsychiatry Neuropsychol Behav Neurol 14:219–226
Fisher CA, Sewell K, Brown A, Churchyard A (2014) Aggression in Huntington’s disease: a systematic review of rates of aggression and treatment methods. J Huntingtons Dis 3:319–332
Schubert R, Frank F, Nagelmann N et al (2016) Neuroimaging of a minipig model of Huntington’s disease: feasibility of volumetric, diffusion-weighted and spectroscopic assessments. J Neurosci Methods 265:46–55
Wang H, Suh JW, Das SR et al (2012) Multi-atlas segmentation with joint label fusion. IEEE Trans Pattern Anal Mach Intell 35:611–623
Wang H, Yushkevich PA (2013) Multi-atlas segmentation with joint label fusion and corrective learning-an open source implementation. Front Neuroinform 7:27
Saikali S, Meurice P, Sauleau P et al (2010) A three-dimensional digital segmented and deformable brain atlas of the domestic pig. J Neurosci Methods 192:102–109
Ghayoor A, Vaidya JG, Johnson HJ (2013) Development of a novel constellation based landmark detection algorithm. Proc SPIE 86693:F-6
Jones DK, Horsfield MA, Simmons A (1999) Optimal strategies for measuring diffusion in anisotropic systems by magnetic resonance imaging. Magn Reson Med 42:515–525
Schramke S, Schubert R, Frank F et al (2015) The Libechov minipig as a large animal model for preclinical research in Huntington’s disease – thoughts and perspectives. Cesk Slov Neurol 78(Suppl 2):2S55–2S60
Acknowledgments
The work reported in this chapter was conducted between 2009 and 2017. The longitudinal phenotyping study including MRI protocol development was funded by the CHDI Foundation (www.chdifoundation.org) from 2012 to 2015; we particularly appreciate the continuous input and encouragement provided by David Howland from CHDI Foundation. Continuation of the work was supported by generous private donations of HD families who want to remain anonymous, but have no conflicts of interest in this project or biomedical research in general. HD families who supported the projects conducted at the George-Huntington -Institute provided additional funding for this work. We thank Jan Motlik, Zdenka Ellederova, Monika Baxa, and Stefan Juhas from the Research Center PIGMOD & Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic, for support of the project and for providing the wt and tgHD minipigs for this study. The imaging analysis performed with minipigs was supported by Hans J. Johnson from the University of Iowa. We are very grateful for the intense support this project has received for many years from the Faculty of Medicine at the University of Muenster, Germany, particularly (1) the Central Animal Facility represented by Stefan Schlatt, Jens Ehmcke, Sandra Stoeppeler, and the whole team at the facility; (2) Department of Clinical Radiology with Cornelius Faber, Walter Heindel, Nina Nagelmann, and Harald Kugel; (3) Martin Luecke from the Office for Animal Care; and (4) E. Bernd Ringelstein and Michael Deppe from the Department of Neurology. We also gratefully acknowledge the input provided by Nicole Kemper, Ute Radespiel, and Michael Wendt, University of Veterinary Medicine Hannover, Germany. Some elements of this chapter are part of the dissertations of Verena Schuldenzucker, Sarah Schramke, Lorena Rieke, Tamara Matheis, Maike Wirsig, Eva Hölzner, Robin Schubert, and Frauke Frank.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Reilmann, R., Schuldenzucker, V. (2018). Minipigs as a Large-Brained Animal Model for Huntington’s Disease: From Behavior and Imaging to Gene Therapy. In: Precious, S., Rosser, A., Dunnett, S. (eds) Huntington’s Disease. Methods in Molecular Biology, vol 1780. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7825-0_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7825-0_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7824-3
Online ISBN: 978-1-4939-7825-0
eBook Packages: Springer Protocols