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A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms

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Book cover Colorectal Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1765))

Abstract

Noninvasive screening methods for the detection of colorectal cancers (CRC) at curable stages rely on the identification of specific biomarkers. Our group has shown that mRNA stool assays represent a powerful and robust approach for the prediction of colorectal neoplasms. In this methodological chapter, we describe the procedures to isolate good quality stool RNA and the steps to evaluate the levels of specific host mRNA markers such as ITGA6, MYC, and GADD45B using TaqMan-based quantitative and droplet digital PCR approaches.

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Acknowledgments

The original studies were supported by Canadian Institutes of Health Research grants MOP 97836 and PPP 133373. JFB is the recipient of the Canada Chair in Intestinal Physiopathology and a member of the FRQS-funded Centre de Recherche of the Centre Hospitalier Universitaire de Sherbrooke.

The authors thank Bio-Rad Laboratories Canada and Dr. Katia Nadeau for the prolonged trial period with the ddPCR equipment and Dr. Sean C. Taylor for scientific advice.

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Authors and Affiliations

  1. Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada

    Elizabeth Herring, Eric Tremblay & Jean-François Beaulieu

  2. Department of Gastroenterology, Hamamatsu Medical Center, Hamamatsu, Japan

    Shigeru Kanaoka

Authors
  1. Elizabeth Herring
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  2. Shigeru Kanaoka
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  3. Eric Tremblay
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  4. Jean-François Beaulieu
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Corresponding author

Correspondence to Jean-François Beaulieu .

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Editors and Affiliations

  1. Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Science, Université de Sherbrooke, Sherbrooke, Québec, Canada

    Jean-François Beaulieu

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Herring, E., Kanaoka, S., Tremblay, E., Beaulieu, JF. (2018). A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms. In: Beaulieu, JF. (eds) Colorectal Cancer. Methods in Molecular Biology, vol 1765. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7765-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7765-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7764-2

  • Online ISBN: 978-1-4939-7765-9

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