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Colorectal Cancer

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Jean-François Beaulieu

    1. Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Science, Université de Sherbrooke, Sherbrooke, Canada

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1765)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Molecular Basis of Colorectal Cancer

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Cell Line Models of Molecular Subtypes of Colorectal Cancer

      • Jennifer K. Mooi, Ian Y. Luk, John M. Mariadason
      Pages 3-26

    3. Dissecting Oncogenic RTK Pathways in Colorectal Cancer Initiation and Progression

      • Stephen McManus, Walid Chababi, Dominique Arsenault, Claire M. Dubois, Caroline Saucier
      Pages 27-42

    4. Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation

      • Salah Boudjadi, Julie C. Carrier, Jean-François Beaulieu
      Pages 43-56

    5. Identification and Functional Analysis of Gene Regulatory Sequences Interacting with Colorectal Tumor Suppressors

      • Katja Dahlgaard, Jesper Thorvald Troelsen
      Pages 57-77

    6. Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinogenesis

      • Martine Chevillard-Briet, Fabrice Escaffit
      Pages 79-85

    7. The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer-Associated Fibroblasts

      • Rahul Bhome, Massimiliano Mellone, Katherine Emo, Gareth J. Thomas, A. Emre Sayan, Alex H. Mirnezami
      Pages 87-98

    8. Methods for Assessing Apoptosis and Anoikis in Normal Intestine/Colon and Colorectal Cancer

      • Pierre H. Vachon
      Pages 99-137

    9. Molecular Analysis of the Microbiome in Colorectal Cancer

      • Fiona Clegg, Susan H. Berry, Richard Hansen, Georgina L. Hold
      Pages 139-153

    10. Proteomics Analysis of Colorectal Cancer Cells

      • Anaïs Chauvin, François-Michel Boisvert
      Pages 155-166

    11. Autophagic Flux Assessment in Colorectal Cancer Cells

      • Annie Lauzier, Steve Jean
      Pages 167-175

  3. New Developments in Colorectal Cancer Diagnostics and Screening

    1. Front Matter

      Pages 177-177
      Download chapter PDF

    2. Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistry

      • Sanne ten Hoorn, Anne Trinh, Joan de Jong, Lianne Koens, Louis Vermeulen
      Pages 179-191

    3. Stool DNA Integrity Method for Colorectal Cancer Detection

      • Claudia Rengucci, Giulia De Maio, Maura Menghi, Daniele Calistri
      Pages 193-202

    4. RT-qPCR for Fecal Mature MicroRNA Quantification and Validation

      • Farid E. Ahmed, Nancy C. Ahmed, Mostafa M. Gouda, Paul W. Vos, Chris Bonnerup
      Pages 203-215

    5. A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasms

      • Elizabeth Herring, Shigeru Kanaoka, Eric Tremblay, Jean-François Beaulieu
      Pages 217-227

    6. Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling

      • Danijel Djukovic, Jiangjiang Zhang, Daniel Raftery
      Pages 229-240

    7. Proteomic Profiling for Colorectal Cancer Biomarker Discovery

      • Paula Álvarez-Chaver, Loretta De Chiara, Vicenta Soledad Martínez-Zorzano
      Pages 241-269

    8. Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution

      • Diane Mege, Laurence Panicot-Dubois, Christophe Dubois
      Pages 271-277
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Keywords

About this book

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer. The book’s chapters are divided into three parts: the chapters in Part One examine techniques used to study the molecular mechanisms in colorectal cancer development and progression. Part Two focuses on the innovative tools used to diagnose and detect cancer lesions in the early stages of cancer. Finally, Part Three discusses recent advancements in treating colorectal tumors and identifying new therapeutic molecules for treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Colorectal Cancer: Methods and Protocols is a valuable resource for any scientist and researcher interested in this field of study.

Editors and Affiliations

  • Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health Science, Université de Sherbrooke, Sherbrooke, Canada

    Jean-François Beaulieu

Bibliographic Information

  • Book Title: Colorectal Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Jean-François Beaulieu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7765-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7764-2Published: 28 March 2018

  • Softcover ISBN: 978-1-4939-9278-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7765-9Published: 27 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 353

  • Number of Illustrations: 39 b/w illustrations, 17 illustrations in colour

  • Topics: Cancer Research

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