Abstract
Time-lapse imaging of roots is highly suitable for depicting gravitropic growth behaviors. However, roots may show faster or slower bending kinetics when compared to control as a result of differences in overall root growth. Accordingly, conditions that cause differential organ growth require growth rate normalization to compare gravitropic curvature. Here, we describe a simple normalization method for gravitropic root growth evaluation. We exemplify this method by exposing seedlings to distinct environmental conditions or disturbing the cellular auxin contents. This data shows that the method is suitable to discriminate between gravitropic and overall organ growth defects.
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Acknowledgments
This work was supported by the Vienna Science and Technology Fund (WWTF) (Vienna Research Group project to J.K.-V.), Austrian Science Fund (FWF) (Projects: P29754 to J.K.-V. and T-728-B16 to E.F.), and the European Research Council (ERC) (Starting Grant 639478-AuxinER to J.K-V.).
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Schöller, M., Sarkel, E., Kleine-Vehn, J., Feraru, E. (2018). Growth Rate Normalization Method to Assess Gravitropic Root Growth. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7747-5_15
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DOI: https://doi.org/10.1007/978-1-4939-7747-5_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7746-8
Online ISBN: 978-1-4939-7747-5
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