Abstract
Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated “wavy” surfaces.
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18 March 2023
The original version of this chapter was incorrectly published with the following note
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Acknowledgments
We gratefully acknowledge financial support from the Groupement des Entreprises Françaises dans la Lutte contre le Cancer (GEFLUC) Ile-de-France, the Région Ile-de-France Domaine d’Intérêt Majeur (DIM) Nano-K, the Association pour la Recherche sur le Cancer (ARC), the EU cofund PRESTIGE post-doc program, the EU cofund IC-3i PhD program, and the Fondation Pierre-Gilles de Gennes. The “Biology-inspired Physics at MesoScales” group is a member of the CelTisPhyBio Labex and of the Institut Pierre-Gilles de Gennes. It is a pleasure to thank Mohamed El Beheiry for his help in the 3D processing of our images.
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Duclos, G. et al. (2018). Controlling Confinement and Topology to Study Collective Cell Behaviors. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_28
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DOI: https://doi.org/10.1007/978-1-4939-7701-7_28
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