Abstract
Oxidation of membrane lipids by reactive oxygen species primarily generates lipid peroxides, from which various carbonyls, i.e., aldehydes and ketones, are formed. Among them, those with a carbonyl-conjugated C-C double bond have significant biological functions and are designated as reactive carbonyl species (RCS). A dozen kinds of RCS occurring in plant cells have a broad spectrum of reactivity and biological effects, depending on the structure. Several RCS have been recently found to activate caspase-like proteases in plants, thereby initiating PCD. Comprehensive and quantitative RCS analysis method using conventional HPLC is illustrated.
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Mano J (2012) Reactive carbonyl species: their production from lipid peroxides, action in environmental stress, and the detoxification mechanism. Plant Physiol Biochem 59:90–97
Farmer EE, Mueller MJ (2013) ROS-mediated lipid peroxidation and RES-activated signaling. Annu Rev Plant Biol 64:429–450
Esterbauer H, Schauer R, Zollner JH (1991) Chemistry and biochemistry of 4-hydroxynonenal, malondialdehyde and related aldehydes. Free Radic Biol Med 11:81–128
Reynolds T (1977) Comparative effects of aliphatic compounds on inhibition of lettuce fruit germination. Ann Bot 41:637–648
Biswas MS, Mano J (2015) Lipid peroxide-derived short-chain carbonyls mediate hydrogen peroxide-induced and salt-induced programmed cell death in plants. Plant Physiol 168:885–898
Srivastava S, Brychkva G, Yarmolinsky D, Soltabyeva A, Samani T, Sagi M (2017) Aldehyde oxidase 4 plays a critical role in delaying silique senescence by catalyzing aldehyde detoxification. Plant Physiol 173:1977–1997
Kai H, Hirashima K, Matsuda O et al (2012) Thermotolerant cyclamen with reduced acrolein and methyl vinyl ketone. J Exp Bot 63:4143–4150
Matsui K, Sugimoto K, Kakyumyan P et al (2009) Volatile oxylipins and related compounds formed under stress in plants. In: Armstrong D (ed) Methods in molecular biology ‘Lipidomics’. Humana Press, Totowa, pp 17–28
Mano J, Khorobrykh S, Matsui K et al (2014) Acrolein is formed from trienoic fatty acids in chloroplasts: a targeted metabolomics approach. Plant Biotechnol 31:535–544
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Mano, J., Biswas, M.S. (2018). Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress. In: De Gara, L., Locato, V. (eds) Plant Programmed Cell Death. Methods in Molecular Biology, vol 1743. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7668-3_11
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DOI: https://doi.org/10.1007/978-1-4939-7668-3_11
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