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Modeling Glioma with Human Embryonic Stem Cell-Derived Neural Lineages

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Glioblastoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1741))

Abstract

Gliomas are malignant primary tumors of the central nervous system. Their cell-of-origin is thought to be a neural progenitor or stem cell that acquires mutations leading to oncogenic transformation. Thanks to advances in human stem cell biology, it has become possible to derive human cell types that represent putative cells-of-origin in vitro and model the gliomagenesis process by systematically introducing genetic alterations in these human cells. Here, we present methods to derive human neural stem cells (NSCs) from human embryonic stem cells (hESCs). Because these NSCs are genetically unmodified at baseline, they can be used as a cellular platform to study the effects of individual oncogenic hits in a well-controlled manner in the backdrop of a human genetic background. We also present some key applications of these NSCs, which include their transduction with lentiviral vectors, their neuroglial differentiation and xenografting methods into immunocompromised mice to assess in vivo behavior.

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Authors and Affiliations

  1. Department of Neurosurgery, NYU School of Medicine, New York, NY, USA

    Aram S. Modrek, Jod Prado, Devin Bready, Joravar Dhaliwal, Danielle Golub & Dimitris G. Placantonakis

  2. Kimmel Center for Stem Cell Biology, NYU School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  3. Laura and Isaac Perlmutter Cancer Center, NYU School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  4. Brain Tumor Center, NYU School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

  5. Neuroscience Institute, NYU School of Medicine, New York, NY, USA

    Dimitris G. Placantonakis

Authors
  1. Aram S. Modrek
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  2. Jod Prado
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  3. Devin Bready
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  4. Joravar Dhaliwal
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  5. Danielle Golub
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  6. Dimitris G. Placantonakis
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Corresponding author

Correspondence to Dimitris G. Placantonakis .

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Editors and Affiliations

  1. Department of Neurosurgery, NYU School of Medicine, New York, New York, USA

    Dimitris G. Placantonakis

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Modrek, A.S., Prado, J., Bready, D., Dhaliwal, J., Golub, D., Placantonakis, D.G. (2018). Modeling Glioma with Human Embryonic Stem Cell-Derived Neural Lineages. In: Placantonakis, D. (eds) Glioblastoma. Methods in Molecular Biology, vol 1741. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7659-1_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7659-1_19

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