Abstract
The genetic code expansion strategy has become an elegant method for site-specific incorporation of noncanonical amino acids with diverse functionalities into proteins of interest in bacteria, yeast, mammalian cells, and even animals. This technique allows precise labeling as well as manipulation of a given protein to dissect its physiological and/or pathological roles under living conditions. Here, we demonstrate the extension of a recently emerged pyrrolysine-based genetic code expansion strategy for encoding noncanonical amino acids into enteric bacterial pathogens.
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Acknowledgment
This work was supported by the National Key Basic Research Foundation of China (2012CB917301), National Natural Science Foundation of China (21225206, 21521003 and 21432002). We thank the support from Peking University Principal Foundation.
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Zheng, H., Lin, S., Chen, P.R. (2018). Genetic Code Expansion in Enteric Bacterial Pathogens. In: Lemke, E. (eds) Noncanonical Amino Acids. Methods in Molecular Biology, vol 1728. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7574-7_6
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DOI: https://doi.org/10.1007/978-1-4939-7574-7_6
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