Protein NMR

1. Front Matter

   Pages i-xiii

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2. NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology

   • Caitlin M. Quinn, Mingzhang Wang, Tatyana Polenova

   Pages 1-35

3. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-State NMR of Microcrystalline and Membrane-Bound Proteins

   • T. Gopinath, Gianluigi Veglia

   Pages 37-53

4. Afterglow Solid-State NMR Spectroscopy

   • Gili Abramov, Nathaniel J. Traaseth

   Pages 55-66

5. Filamentous Bacteriophage Viruses: Preparation, Magic-Angle Spinning Solid-State NMR Experiments, and Structure Determination

   • Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, Amir Goldbourt

   Pages 67-97

6. Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein Interactions with Curved Membranes

   • Erin R. Tyndall, Fang Tian

   Pages 99-109

7. Rapid Prediction of Multi-dimensional NMR Data Sets Using FANDAS

   • Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, Marc Baldus

   Pages 111-132

8. Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules

   • Boris Itin, Ivan V. Sergeyev

   Pages 133-154

9. In-Vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules

   • Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, Christian Hilty

   Pages 155-168

10. Determination of Protein ps-ns Motions by High-Resolution Relaxometry

    • Samuel F. Cousin, Pavel Kadeřávek, Nicolas Bolik-Coulon, Fabien Ferrage

    Pages 169-203

11. Characterizing Protein Dynamics with NMR R _1ρ Relaxation Experiments

    • Francesca Massi, Jeffrey W. Peng

    Pages 205-221

12. CPMG Experiments for Protein Minor Conformer Structure Determination

    • Anusha B. Gopalan, D. Flemming Hansen, Pramodh Vallurupalli

    Pages 223-242

13. Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR

    • Vincenzo Venditti, Nicolas L. Fawzi

    Pages 243-255

14. From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis

    • Bruce A. Johnson

    Pages 257-310

15. Protein Structure Elucidation from NMR Data with the Program Xplor-NIH

    • Guillermo A. Bermejo, Charles D. Schwieters

    Pages 311-340

16. Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR

    • Mark W. Maciejewski, Adam D. Schuyler, Jeffrey C. Hoch

    Pages 341-352

17. Covariance NMR Processing and Analysis for Protein Assignment

    • Bradley J. Harden, Dominique P. Frueh

    Pages 353-373

18. Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System

    • Wolfgang Peti, Rebecca Page, Evzen Boura, Bartosz Różycki

    Pages 375-389

19. Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist’s Approach to Allostery

    • Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, Giuseppe Melacini

    Pages 391-405

20. High-Efficiency Expression of Yeast-Derived G-Protein Coupled Receptors and 19F Labeling for Dynamical Studies

    • Libin Ye, Alexander P. Orazietti, Aditya Pandey, R. Scott Prosser

    Pages 407-421

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest.  Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.

Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.

• spectroscopy
• magnet technologies
• afterglow experiments
• solid-state NMR
• dynamic nuclear polarization

“The book covers current applications of solid-state and solution nuclear magnetic resonance spectroscopy to study protein structure, dynamics. and interactions. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols with tips to tackle troubleshooting. The book has several high quality illustrations in color or black and white. … The intended audience appears to be students, specialists, and those who are interested in NMR spectroscopy.” (Omer Iqbal, Doody's Book Reviews, April, 2018)

• Department of Chemistry and Biochemistry, The City College of New York, New York, USA

  Ranajeet Ghose

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