Abstract
Transcriptional control of gene expression in skeletal muscle cell is involved in different processes ranging from muscle formation to regeneration. The identification of an increasing number of transcription factors, co-factors, and histone modifications has been greatly advanced by methods that allow studies of genome-wide chromatin-protein interactions. Chromatin immunoprecipitation with massively parallel DNA sequencing, or ChIP-seq, is a powerful tool for identifying binding sites of TFs/co-factors and histone modifications. The major steps of this technique involve immunoprecipitation of fragmented chromatin, followed by high-throughput sequencing to identify the protein bound regions genome-wide. Here, in this protocol, we will illustrate how the entire ChIP-seq is performed using global H3K27ac profiling in myoblast cells as an example.
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Acknowledgment
The work is substantially supported by General Research Funds (GRF) from the Research Grants Council (RGC) of the Hong Kong Special Administrative Region [14102315 and 14113514 to H.S.; 14133016, 14100415, 14116014, and 476113 to H.W.]; Focused Innovations Scheme: Scheme B to H.S. [Project Code: 1907307]; RGC Collaborative Research Fund (CRF) from RGC [Project Code: C6015- 14G to H.S. and H.W.].
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So, K.K., Peng, X.L., Sun, H., Wang, H. (2017). Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells. In: Ryall, J. (eds) Skeletal Muscle Development. Methods in Molecular Biology, vol 1668. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7283-8_2
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DOI: https://doi.org/10.1007/978-1-4939-7283-8_2
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