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Analysis of Phragmoplast Kinetics During Plant Cytokinesis

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Plant Protein Secretion

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1662))

Abstract

In plants, the partitioning of daughter cells during cytokinesis is achieved via physical insertion of a membranous cell plate within the dividing parent cell. It is a cellular process of extensive protein secretion and membrane trafficking toward the plane of cell division and the cytoskeleton is an important facilitator of this process. A specialized cytoskeletal array termed phragmoplast expands centrifugally throughout cytokinesis and directs, mostly Golgi-derived vesicles that ultimately fuse to form the developing cell plate. The function of the phragmoplast in guiding cell plate synthesis has strongly motivated many scientists to monitor its dynamic behavior. In this chapter, we present an overview of basic principles and methods concerning the live imaging of cytokinetic plant cells using confocal laser scanning microscopy (CLSM) and the analysis of phragmoplast expansion.

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Acknowledgments

Research in the laboratory of S.M. is provided by the Deutsche Forschungsgemeinschaft (grants MU3133/1-1, MU3133/3-1 and SFB1101).

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Authors and Affiliations

  1. Developmental Genetics, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany

    Pantelis Livanos & Sabine Müller

  2. Cellular Nanoscience, Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle 32, 72076, Tübingen, Germany

    Mayank Chugh

Authors
  1. Pantelis Livanos
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  2. Mayank Chugh
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  3. Sabine Müller
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Corresponding author

Correspondence to Sabine Müller .

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Editors and Affiliations

  1. School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China

    Liwen Jiang

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Livanos, P., Chugh, M., Müller, S. (2017). Analysis of Phragmoplast Kinetics During Plant Cytokinesis. In: Jiang, L. (eds) Plant Protein Secretion. Methods in Molecular Biology, vol 1662. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7262-3_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7262-3_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7261-6

  • Online ISBN: 978-1-4939-7262-3

  • eBook Packages: Springer Protocols

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