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Secreted Reporters for Monitoring Multiple Promoter Function

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Mammalian Synthetic Promoters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1651))

Abstract

Secreted reporter proteins are reliable modalities for monitoring of different biological processes, which can be measured longitudinally in conditioned medium of cultured cells or body fluids such as blood and urine, ex vivo. In this chapter, we will explore established secreted reporters and their applications and limitations for monitoring of promoter function. We will also describe both cell-based and blood-based assays for detecting three commonly used reporters: secreted alkaline phosphatase (SEAP ), Gaussia luciferase (Gluc), and Vargula luciferase (Vluc).

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Author notes

  1. Ghazal Lashgari and Rami S. Kantar contributed equally to this work.

Authors and Affiliations

  1. Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA

    Ghazal Lashgari, Rami S. Kantar & Bakhos A. Tannous Ph.D.

  2. Program in Neuroscience, Harvard Medical School, Boston, MA, USA

    Ghazal Lashgari, Rami S. Kantar & Bakhos A. Tannous Ph.D.

  3. Neuroscience Center, Massachusetts General Hospital, Building 149, 13th Street, Charlestown, MA, 02129, USA

    Bakhos A. Tannous Ph.D.

Authors
  1. Ghazal Lashgari
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  2. Rami S. Kantar
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  3. Bakhos A. Tannous Ph.D.
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Corresponding author

Correspondence to Bakhos A. Tannous Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, UK

    David Gould

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Lashgari, G., Kantar, R.S., Tannous, B.A. (2017). Secreted Reporters for Monitoring Multiple Promoter Function. In: Gould, D. (eds) Mammalian Synthetic Promoters. Methods in Molecular Biology, vol 1651. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7223-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7223-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-7221-0

  • Online ISBN: 978-1-4939-7223-4

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