Abstract
Immunoglobulins (Igs)—or antibodies (Ab)—are important to combat foreign pathogens but also to the immune system homeostasis. We developed the AID−/−μS−/− mouse model devoid of total soluble Igs and suitable to monitor the role of Igs on immune homeostasis. We used this experimental system to uncover a negative feedback control of marginal zone (MZ) and B1 B cells numbers by naturally secreted Igs. We raised AID−/−μS−/− mice in germ-free conditions demonstrating that this effect of natural secreted Igs is independent of the microbiota. Herein, we provide a comprehensive description of the protocols to establish and use the AID−/−μS−/− mice to study the role of total secreted Igs or of different Ig classes. This study involves Igs injections to AID−/−μS−/− mice or establishment of AID−/−μS−/− mixed bone marrow chimeras that provide a powerful system to study AID−/−μS−/− B cells in the presence of stable concentrations of different Ig classes. While we describe flow cytometric and histological methods to analyze MZ and B1 B cell subsets, AID−/−μS−/− mice can be used to study the effects of natural Igs on other B cell subsets or immune cells.
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Mohr, E., Lino, A.C. (2017). Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally Secreted Immunoglobulins. In: Kaveri, S., Bayry, J. (eds) Natural Antibodies. Methods in Molecular Biology, vol 1643. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7180-0_7
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DOI: https://doi.org/10.1007/978-1-4939-7180-0_7
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