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Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells

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Vaccines for Invasive Fungal Infections

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1625))

Abstract

Dendritic cells are the most potent antigen-presenting cells, and are critical for the generation of an antigen-specific immune response and protective immunity. These unique features have been applied to dendritic cell-based immunization in a number of disease conditions. Our published results have demonstrated that the immunity induced by intranasal immunization with DNA-transfected dendritic cells results in reduced fungal burden, and alleviated lung tissue damage in a mouse model of pulmonary fungal infection. In this article, approaches for the preparation and characterization of DNA-transfected dendritic cells and intranasal immunization in mice are described.

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Acknowledgments

The author acknowledges funding support from the San Antonio Area Foundation, San Antonio, TX (2003–2004) and Oklahoma Center for Advancement of Science and Technology, Oklahoma City, OK (2007–2010) for the research conducted on dendritic cell-based immunization for coccidioidomycosis. Editorial support from Ms. Kathy Kyler, Staff Editor at the University of Oklahoma Health Sciences Center, is appreciated.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Oklahoma Health Sciences Center, 1110 N. Stonewall Avenue, Oklahoma City, OK, 73117, USA

    Shanjana Awasthi Ph.D.

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  1. Shanjana Awasthi Ph.D.
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Correspondence to Shanjana Awasthi Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Markus Kalkum

  2. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Margarita Semis

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Awasthi, S. (2017). Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells. In: Kalkum, M., Semis, M. (eds) Vaccines for Invasive Fungal Infections. Methods in Molecular Biology, vol 1625. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7104-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7104-6_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7103-9

  • Online ISBN: 978-1-4939-7104-6

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