Abstract
The microbial assessment of potable/drinking water is done to ensure that the resource is free of fecal contamination indicators or waterborne pathogens. Culture-based methods for verifying the microbial safety are limited in the sense that a standard volume of water is generally tested for only one indicator (family) or pathogen.
In this work, we describe a membrane filtration-based molecular microbiology method, CRENAME (Concentration Recovery Extraction of Nucleic Acids and Molecular Enrichment), exploiting molecular enrichment by whole genome amplification (WGA) to yield, in less than 4 h, a nucleic acid preparation which can be repetitively tested by real-time PCR for example, to provide multiparametric presence/absence tests (1 colony forming unit or microbial particle per standard volume of 100-1000 mL) for bacterial or protozoan parasite cells or particles susceptible to contaminate potable/drinking water.
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Acknowledgments
During her doctoral thesis, Andrée F. Maheux held a scholarship from the Nasivvik Center for Inuit Health and Changing Environment (Canadian Institutes of Health Research) and the work was supported in part by the Canada Foundation for Innovation.
Author contributions: L. Bissonnette has written the chapter in collaboration with A.F. Maheux, while M.G. Bergeron was responsible for its revision.
Conflicts of Interests: The authors declare having no conflict of interest.
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Bissonnette, L., Maheux, A.F., Bergeron, M.G. (2017). CRENAME, A Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 1620. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-7060-5_9
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DOI: https://doi.org/10.1007/978-1-4939-7060-5_9
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