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Plant Genomics pp 267–285Cite as

Studying Protein–Protein Interactions In Planta Using Advanced Fluorescence Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1610))

Abstract

The formation of protein complexes through direct protein–protein interaction is essential for virtually every biological process, and accordingly the ability to determine the interaction properties of specific proteins is important to understand these processes. Förster resonance energy transfer (FRET) measurements are state-of-the-art confocal fluorescence microscopy- and imaging-based techniques that allow the analysis of protein interactions in vivo and in planta, in specific compartments of single cells or tissues. Here we provide a step-by-step guide to perform FRET measurements by acceptor photobleaching (APB) and fluorescence lifetime imaging microscopy (FLIM) in the plant expression system Nicotiana benthamiana.

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Acknowledgments

M. S. and R. S. thank Petra Žádníková and Maike Breiden for helpful comments and discussion of the manuscript and the CAi of the HHU for technical support. This work was funded by the D. F. G. through the Exc1028 (CEPLAS).

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Authors and Affiliations

  1. Institute for Developmental Genetics, Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany

    Marc Somssich

  2. School of Biosciences, University of Melbourne, Melbourne, VIC, Australia

    Marc Somssich

  3. Institute for Developmental Genetics, Cluster of Excellence on Plant Sciences (CEPLAS), and Center for Advanced Imaging (CAi), Heinrich Heine University, Universitätsstr. 1, 40225, Düsseldorf, Germany

    Rüdiger Simon

Authors
  1. Marc Somssich
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  2. Rüdiger Simon
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Correspondence to Rüdiger Simon .

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Editors and Affiliations

  1. Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Vienna, Austria

    Wolfgang Busch

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Somssich, M., Simon, R. (2017). Studying Protein–Protein Interactions In Planta Using Advanced Fluorescence Microscopy. In: Busch, W. (eds) Plant Genomics. Methods in Molecular Biology, vol 1610. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7003-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7003-2_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7001-8

  • Online ISBN: 978-1-4939-7003-2

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