Abstract
Nonpathogenic fowl adenoviruses (FAdVs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential DNA sequences in their genomes. We describe the generation of FAdV-9-based vaccine platforms by targeted homologous recombination in an infectious clone (pPacFAdV-9 or wild type FAdmid) containing the entire viral genome in a cosmid vector. The viral DNA is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chicken hepatoma cell line (CH-SAH). Virus is harvested, propagated, and verified for foreign gene expression.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wold WS, Toth K (2013) Adenovirus vectors for gene therapy, vaccination and cancer gene therapy. Curr Gene Ther 13:421–433
Pei Y, Griffin B, de Jong J et al (2015) Rapid generation of fowl adenovirus 9 vectors. J Virol Methods 223:75–81
Corredor JC, Nagy E (2011) Antibody response and virus shedding of chickens inoculated with left end deleted fowl adenovirus 9-based recombinant viruses. Avian Dis 55:443–446
Corredor JC, Nagy E (2010) The non-essential left end region of the fowl adenovirus 9 genome is suitable for foreign gene insertion/replacement. Virus Res 149:167–174
Cherenova LV, Logunov DY, Shashkova EV et al (2004) Recombinant avian adenovirus CELO expressing the human interleukin-2: characterization in vitro, in ovo and in vivo. Virus Res 100:257–261
Francois A, Chevalier C, Delmas B et al (2004) Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens. Vaccine 22:2351–2360
Johnson MA, Pooley C, Ignjatovic J et al (2003) A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus. Vaccine 21:2730–2736
Sheppard M, Werner W, Tsatas E et al (1998) Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease. Arch Virol 143:915–930
Shashkova EV, Cherenova LV, Kazansky DB et al (2005) Avian adenovirus vector CELO-TK displays anticancer activity in human cancer cells and suppresses established murine melanoma tumors. Cancer Gene Ther 12:617–626
Ojkic D, Nagy E (2001) The long repeat region is dispensable for fowl adenovirus replication in vitro. Virology 283:197–206
Corredor JC, Nagy E (2010) A region at the left end of the fowl adenovirus 9 genome that is non-essential in vitro has consequences in vivo. J Gen Virol 91:51–58
Chartier C, Degryse E, Gantzer M et al (1996) Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli. J Virol 70:4805–4810
Michou AI, Lehrmann H, Saltik M et al (1999) Mutational analysis of the avian adenovirus CELO, which provides a basis for gene delivery vectors. J Virol 73:1399–1410
Ojkic D, Nagy E (2000) The complete nucleotide sequence of fowl adenovirus type 8. J Gen Virol 81:1833–1837
McVey D, Zuber M, Ettyreddy D et al (2002) Rapid construction of adenoviral vectors by lambda phage genetics. J Virol 76:3670–3677
Deng L, Sharif S, Nagy E (2013) Oral inoculation of chickens with a candidate fowl adenovirus 9 vector. Clin Vaccine Immunol 20:1189–1196
Gay M, Castro F, Paz G et al. (2015) Development of a vectorized fowl adenovirus vaccine expressing the hemagglutinin gene of influenza virus subtype H5. 9th International Symposium on avian influenza. Athens, Georgia, USA. April 12–15, 2015
Green MR, Sambrook J (2012) Isolation and quantification of DNA. In: Inglis J, Boyle A, Gann A (eds) Molecular cloning: a laboratory manual, Fourth edn, vol 1. Cold Spring Harbor laboratory Press, Cold Spring Harbor, NY, pp 11–71
Green MR, Sambrook J (2012) Cloning and transformation with plasmid vectors. In: Inglis J, Boyle A, Gann A (eds) Molecular cloning: a laboratory manual, Fourth edn, vol 1. Cold Spring Harbor laboratory Press, Cold Spring Harbor, NY, pp 157–258
Green MR, Sambrook J (2012) Expressing cloned genes for protein production, purification, and analysis. In: Inglis J, Boyle A, Gann A (eds) Molecular cloning: a laboratory manual, Fourth edn, vol 1. Cold Spring Harbor laboratory Press, Cold Spring Harbor, NY, pp 1483–1634
Vincze T, Posfai J, Roberts RJ (2003) NEBcutter: a program to cleave DNA with restriction enzymes. Nucleic Acids Res 31:3688–3691
Alexander HS, Huber P, Cao J et al (1998) Growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. J Virol Methods 74:9–14
Wickham TJ, Davis T, Granados RR et al (1991) Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection. Biotechnol Lett 13:483–488
Acknowledgments
This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canadian Poultry Research Council (CPRC), and the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA) to É.N.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Corredor, J.C., Pei, Y., Nagy, É. (2017). Fowl Adenovirus-Based Vaccine Platform. In: Ferran, M., Skuse, G. (eds) Recombinant Virus Vaccines. Methods in Molecular Biology, vol 1581. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6869-5_3
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6869-5_3
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6867-1
Online ISBN: 978-1-4939-6869-5
eBook Packages: Springer Protocols