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Microbial Manipulation of Auxins and Cytokinins in Plants

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Auxins and Cytokinins in Plant Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1569))

Abstract

Microbial associations with plants are crucial for the survival of both the partners. Beside other ways of establishing such associations, phytohormones enjoy a key role in plant–microbe interactions from initial dialog between the two to the establishment of a viable partnership. Cytokinins (CKs) and IAA are among the five classical groups of phytohormones implicated in plant immune response, early signaling, and deciding the fate of interactions between plant and microbes. Here we describe a method to study modulation of Cks and IAA in plant under the influence of a pathogenic bacterium, Pseudomonas syringae tomato DC3000. A method for inoculating bacteria on host plant and subsequent determination of Cks and IAA through HPLC-ESI-MS/MS is described.

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Author information

Authors and Affiliations

  1. Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan, 23200, Pakistan

    Anwar Hussain

  2. Department of Environmental Science, Islamic International University, Islamabad, Sector H-10, Islamabad, Pakistan

    Ihsan Ullah

  3. Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan

    Shahida Hasnain

Authors
  1. Anwar Hussain
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  2. Ihsan Ullah
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  3. Shahida Hasnain
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Corresponding author

Correspondence to Anwar Hussain .

Editor information

Editors and Affiliations

  1. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    Thomas Dandekar

  2. Deptartment of Bioinformatics, Biocenter University of Würzburg Am Hubland Würzburg Germany, Julius-Maximilians-Universität Würzburg, Wurzburg, Germany

    Muhammad Naseem

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Hussain, A., Ullah, I., Hasnain, S. (2017). Microbial Manipulation of Auxins and Cytokinins in Plants. In: Dandekar, T., Naseem, M. (eds) Auxins and Cytokinins in Plant Biology. Methods in Molecular Biology, vol 1569. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6831-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-6831-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6829-9

  • Online ISBN: 978-1-4939-6831-2

  • eBook Packages: Springer Protocols

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