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Muscle Stem Cells

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Eusebio Perdiguero

    1. Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. DDW Cornelison

    1. Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1556)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. Overview

    1. Front Matter

      Pages 1-1
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    2. Muscle Stem Cells: A Model System for Adult Stem Cell Biology

      • DDW Cornelison, Eusebio Perdiguero
      Pages 3-19

  3. Muscle Stem Cells (Satellite cells)

    1. Front Matter

      Pages 21-21
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    2. Isolation of Muscle Stem Cells from Mouse Skeletal Muscle

      • Barbara Gayraud-Morel, Francesca Pala, Hiroshi Sakai, Shahragim Tajbakhsh
      Pages 23-39

    3. Primary Mouse Myoblast Purification using Magnetic Cell Separation

      • Marie Claude Sincennes, Yu Xin Wang, Michael A. Rudnicki
      Pages 41-50

    4. Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cells

      • Pascal Stuelsatz, Paul Keire, Zipora Yablonka-Reuveni
      Pages 51-102

    5. Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors

      • Guillaume Lavergne, Cedric Soler, Monika Zmojdzian, Krzysztof Jagla
      Pages 103-116

    6. Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells

      • Phong D. Nguyen, Peter D. Currie
      Pages 117-125

  4. Other Muscle Interstitial Stem Cells

    1. Front Matter

      Pages 127-127
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    2. Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle

      • Francesco Saverio Tedesco, Louise A. Moyle, Eusebio Perdiguero
      Pages 129-147

    3. Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle

      • Rossana Tonlorenzi, Giuliana Rossi, Graziella Messina
      Pages 149-177

    4. Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

      • Marcela Low, Christine Eisner, Fabio Rossi
      Pages 179-189

    5. Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells

      • Sole Gatto, Pier Lorenzo Puri, Barbora Malecova
      Pages 191-219

  5. Assays for Stem Cell Functionality

    1. Front Matter

      Pages 221-221
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    2. Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

      • Matthew Tierney, Alessandra Sacco
      Pages 223-236

    3. Transplantation of Skeletal Muscle Stem Cells

      • Monica N. Hall, John K. Hall, Adam B. Cadwallader, Bradley T. Pawlikowski, Jason D. Doles, Tiffany L. Elston et al.
      Pages 237-244

    4. Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells

      • James G. Ryall
      Pages 245-253

    5. Monitoring Autophagy in Muscle Stem Cells

      • Laura García-Prat, Pura Muñoz-Cánoves, Marta Martínez-Vicente
      Pages 255-280

  6. Cell culture, In Vitro, and In Silico Studies

    1. Front Matter

      Pages 281-281
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Keywords

About this book

This volume provides leading-edge protocols in the study of the molecular and cellular biology of muscle stem cells. Chapters detail current and updated methods for muscle stem cell isolation, culture, molecular analysis, cellular analysis, and reintroduction in vivo as well as protocols for studying myogenic stem cells in non-mammalian model systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Muscle Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Cell Biology Group, Department of Experimental and Health Sciences, University Pompeu Fabra (UPF), Barcelona, Spain

    Eusebio Perdiguero

  • Division of Biological Sciences and Christopher S. Bond Life Sciences Center, University of Missouri, Columbia, USA

    DDW Cornelison

Bibliographic Information

  • Book Title: Muscle Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Eusebio Perdiguero, DDW Cornelison

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6771-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6769-8Published: 02 March 2017

  • Softcover ISBN: 978-1-4939-8296-7Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6771-1Published: 27 February 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 365

  • Topics: Cell Biology

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