Abstract
Compounds that inhibit virulence gene expression in bacterial pathogens have received increasing interest as possible alternatives to the traditional antibiotic treatment of infections. For the human pathogen Staphylococcus aureus, we have developed two simple assays based on reporter gene fusions to central virulence genes that are easily applicable for screening various sources of natural and synthetic peptides for anti-virulence effects. The plate assay is qualitative but simultaneously assesses the effect of gradient concentrations of the investigated compound, whereas the liquid assay is quantitative and can be employed to address whether a compound is acting on the central quorum sensing regulatory system, agr, that controls a large number of virulence genes in S. aureus.
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References
Cegelski L, Marshall GR, Eldridge GR, Hultgren SJ (2008) The biology and future prospects of antivirulence therapies. Nat Rev Microbiol 6:17–27
Novick RP, Geisinger E (2008) Quorum sensing in staphylococci. Annu Rev Genet 42:541–564
Chan PF, Foster SJ (1998) The role of environmental factors in the regulation of virulence-determinant expression in Staphylococcus aureus 8325-4. Microbiology 144:2469–2479
Nielsen A, Nielsen KF, Frees D, Larsen TO, Ingmer H (2010) Method for screening compounds that influence virulence gene expression in Staphylococcus aureus. Antimicrob Agents Chemother 54:509–512
Nielsen A, Mansson M, Wietz M, Varming AN, Phipps RK, Larsen TO, Gram L, Ingmer H (2012) Nigribactin, a novel siderophore from Vibrio nigripulchritudo, modulates Staphylococcus aureus virulence gene expression. Mar Drugs 10:2584–2595
Kjaerulff L, Nielsen A, Mansson M, Gram L, Larsen TO, Ingmer H, Gotfredsen CH (2013) Identification of four new agr quorum sensing-interfering cyclodepsipeptides from a marine Photobacterium. Mar Drugs 11:5051–5062
Mansson M, Nielsen A, Kjærulff L, Gotfredsen CH, Wietz M, Ingmer H, Gram L, Larsen TO (2011) Inhibition of virulence gene expression in Staphylococcus aureus by novel depsipeptides from a marine photobacterium. Mar Drugs 9:2537–2552
Nielsen A, Månsson M, Bojer MS, Gram L, Larsen TO, Novick RP, Frees D, Frøkiær H, Ingmer H (2014) Solonamide B inhibits quorum sensing and reduces Staphylococcus aureus mediated killing of human neutrophils. PLoS One 9:e84992
Geisinger E, Muir TW, Novick RP (2009) agr receptor mutants reveal distinct modes of inhibition by staphylococcal autoinducing peptides. Proc Natl Acad Sci U S A 106:1216–1221
Novick RP, Morse SI (1967) In vivo transmission of drug resistance factors between strains of Staphylococcus aureus. J Exp Med 125:45–59
Novick RP, Ross HF, Projan SJ, Kornblum J, Kreiswirth B, Moghazeh S (1993) Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 12:3967–3975
Horsburgh MJ, Aish JL, White IJ, Shaw L, Lithgow JK, Foster SJ (2002) sigmaB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J Bacteriol 184:5457–5467
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Bojer, M.S., Baldry, M., Ingmer, H. (2017). Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus . In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_28
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DOI: https://doi.org/10.1007/978-1-4939-6737-7_28
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Publisher Name: Humana Press, New York, NY
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