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Carbohydrate Composition Analysis in Xylem

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Xylem

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1544))

Abstract

Gas chromatography-mass spectrometry (GC-MS) is a widely applied technique in carbohydrate composition analysis due to high accuracy and repeatability. As cell walls are the major carbohydrate pool in xylem tissues, the measurement of carbohydrate composition essentially constitutes an analysis of wall residues. Here, we describe the method for quantification of the contents of several common neutral monosaccharides and cellulose, the major components of cell walls in poplar xylem tissues, by GC-MS. The procedures for extraction of cell wall residues from xylem, generation of alditol derivatives, subsequent further separation on gas chromatograph, and detection by mass spectrometer are included.

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References

  1. Watanabe Y, Meents MJ, McDonnell LM, Barkwill S, Sampathkumar A, Cartwright HN, Demura T, Ehrhardt DW, Samuels AL, Mansfield SD (2015) Visualization of cellulose synthases in Arabidopsis secondary cell walls. Science 350:198–203

    Article  CAS  PubMed  Google Scholar 

  2. Somerville C, Bauer S, Brinistool G, Facette M, Hamann T, Milne J, Osborne E, Paradez A, Persson S, Raab T, Vorwerk S, Youngs H (2004) Toward a systems approach to understanding plant-cell walls. Science 306:2206–2211

    Article  CAS  PubMed  Google Scholar 

  3. Burton RA, Gidley MJ, Fincher GB (2010) Heterogeneity in the chemistry, structure and function of plant cell walls. Nat Chem Biol 6:724–732

    Article  CAS  PubMed  Google Scholar 

  4. Scheller HV, Ulvskov P (2010) Hemicelluloses. Annu Rev Plant Biol 61:263–289

    Article  CAS  PubMed  Google Scholar 

  5. Scott TA, Melvin EH (1953) Determination of dextran with anthrone. Anal Biochem 28:1656–1661

    Google Scholar 

  6. Dubois M, Gilees KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Biochem 28:350–356

    CAS  Google Scholar 

  7. Updegraff DM (1969) Semimicro determination of cellulose in biological materials. Anal Biochem 32:420–424

    Article  CAS  PubMed  Google Scholar 

  8. Fry SC (1988) Wall polymer: chemical characterisation. In: Fry SC (ed) The growing plant cell wall: chemical and metabolic analysis. Blackburn, Caldwell, NJ, pp 154–172

    Google Scholar 

  9. Albersheim P, Nevins DJ, English PD, Karr A (1967) A method for the analysis of sugars in plant cell-wall polysaccharides by gas-liquid chromatography. Carbohydr Res 5:340–345

    Article  CAS  Google Scholar 

  10. York WS, Darvill AG, McNeil M, Stevenson TT, Albersheim P (1986) Isolation and characterization of plant cell walls and cell wall components. Methods Enzymol 118:3–40

    Article  CAS  Google Scholar 

  11. Foster CE, Martin TM, Pauly M (2010) Comprehensive compositional analysis of plant cell walls (Lignocellulosic biomass): Part II. Carbohydrates. J Vis Exp 37:e1837

    Google Scholar 

  12. Pettolino FA, Walsh C, Fincher GB, Bacic A (2012) Determining the polysaccharide composition of plant cell walls. Nat Protoc 7:1590–1607

    Article  CAS  PubMed  Google Scholar 

  13. Sweeley CC, Bentley R, Makita M, Weils W (1963) Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Am Chem Soc 85:2497–2505

    Article  CAS  Google Scholar 

  14. Verhaar LAT, Kuster BFM (1981) Liquid chromtography of sugars on silica-based stationary phases. J Chromatogr 220:313–328

    Article  CAS  PubMed  Google Scholar 

  15. Hounsell EF, Davies MJ, Smith KD (1996) Monosaccharide analysis by HPAEC. In: Walker JM (ed) The protein protocols handbook. Humana, Totowa, NJ, pp 635–637

    Chapter  Google Scholar 

  16. Mansfield SD, Kim H, Lu F, Ralph J (2012) Whole plant cell wall characterization using 2D-NMR. Nat Protoc 7:1579–1589

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by the Ministry of Sciences and Technology of China (Grant 2012CB114501) and the National Natural Science Foundation of China (Grants 31530051, 31370310). We thank Dr. Xueqin Song and Prof. Mengzhu Lu (Research Institute of Forestry, Chinese Academy of Forestry) for providing the poplar stems .

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Baocai Zhang & Yihua Zhou

Authors
  1. Baocai Zhang
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  2. Yihua Zhou
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Corresponding author

Correspondence to Yihua Zhou .

Editor information

Editors and Affiliations

  1. Department of Biosciences, Durham University, Durham, United Kingdom

    Miguel de Lucas

  2. Department of Biosciences, Durham University, Durham, United Kingdom

    J. Peter Etchhells

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Zhang, B., Zhou, Y. (2017). Carbohydrate Composition Analysis in Xylem. In: de Lucas, M., Etchhells, J. (eds) Xylem. Methods in Molecular Biology, vol 1544. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6722-3_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6722-3_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6720-9

  • Online ISBN: 978-1-4939-6722-3

  • eBook Packages: Springer Protocols

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