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Quantitative Fluorescence In Situ Hybridization (QFISH)

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Cancer Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1541))

Abstract

Fluorescence in situ hybridization (FISH) has a wide spectrum of applications in current molecular cytogenetic and cancer research. This is a unique technique that can be used for chromosomal DNA analysis in all cell types, at all stages of the cell cycle, and at molecular resolution. Recent developments in microscopy and imaging systems have allowed quantification of digital FISH images (quantitative FISH or QFISH) and have provided a new way for molecular cytogenetic analysis at single-cell level. QFISH can be applied for studying chromosome imbalances in interphase nuclei or metaphase spreads, measuring relative DNA content at chromosomal loci and identifying parental origin of homologous chromosomes. Here, a QFISH protocol suitable for the majority of DNA probes using the popular US National Institute of Health developed ImageJ software is described.

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Acknowledgements

The article is dedicated to Ilia V. Soloviev. I would like to express my gratitude to Prof. Svetlana G Vorsanova and Prof. Yuri B Yurov for helping in the preparation of this chapter. This work was supported by the Russian Science Foundation (Grant #14-35-00060).

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Authors and Affiliations

  1. Mental Health Research Center, Zagorodnoe sh. 2, Moscow, 117152, Russia

    Ivan Y. Iourov

  2. Separated Structural Unit “Clinical Research Institute of Pediatrics” named after Y.E. Veltishev, Russian National Research Medical University named after N.I. Pirogov, Ministry of Health of Russian Federation, Moscow, Russia

    Ivan Y. Iourov

  3. Moscow State University of Psychology and Education, Moscow, Russia

    Ivan Y. Iourov

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  1. Ivan Y. Iourov
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Correspondence to Ivan Y. Iourov .

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Editors and Affiliations

  1. Haematology Division Department of Anatomical and Cellular Pathology, The Chinese University of Hong Kong Prince of Wales Hospital, Shatin, Hong Kong, China

    Thomas S.K. Wan

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Iourov, I.Y. (2017). Quantitative Fluorescence In Situ Hybridization (QFISH). In: Wan, T. (eds) Cancer Cytogenetics. Methods in Molecular Biology, vol 1541. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6703-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-6703-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6701-8

  • Online ISBN: 978-1-4939-6703-2

  • eBook Packages: Springer Protocols

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