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Activity-Based Protein Profiling: From Chemical Novelty to Biomedical Stalwart

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Activity-Based Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1491))

Abstract

Biological systems often respond to environmental changes by rapidly altering the activity of specific enzymes: for example through desequesterization of enzyme activities by dissociation from inhibitors, activation/deactivation through posttranslational modification, or relocation of the enzyme to different organelles. This means that expression levels of enzymes do not necessarily correlate with the activities observed for these enzymes. In this chapter we review some of the approaches used to selectively image only the active sub-populations of given enzymes, the so-called activity-based protein profiling. A focus lies on recent developments that are taking this approach from chemical novelty to biochemical stalwart.

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Author information

Authors and Affiliations

  1. Bio-Organic Synthesis Group, Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC, Leiden, The Netherlands

    Sander I. van Kasteren, Bogdan I. Florea & Herman S. Overkleeft

Authors
  1. Sander I. van Kasteren
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  2. Bogdan I. Florea
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  3. Herman S. Overkleeft
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Corresponding author

Correspondence to Sander I. van Kasteren .

Editor information

Editors and Affiliations

  1. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Herman S. Overkleeft

  2. Bio-Organic Synthesis Group Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands

    Bogdan I. Florea

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van Kasteren, S.I., Florea, B.I., Overkleeft, H.S. (2017). Activity-Based Protein Profiling: From Chemical Novelty to Biomedical Stalwart. In: Overkleeft, H., Florea, B. (eds) Activity-Based Proteomics. Methods in Molecular Biology, vol 1491. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6439-0_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6439-0_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6437-6

  • Online ISBN: 978-1-4939-6439-0

  • eBook Packages: Springer Protocols

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