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  • © 2017

CCN Proteins

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1489)

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Table of contents (46 protocols)

  1. Front Matter

    Pages i-xv
  2. The CCN Proteins: An Overview

    • Masaharu Takigawa
    Pages 1-8
  3. Basic Methods and Protocols

    1. Front Matter

      Pages 9-9
    2. Expression of CCN Genes and Proteins in Human Skin: Methods and Protocols

      • Yilei Cui, Sarah Campbell, Gary J. Fisher
      Pages 21-31
    3. Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 33-41
    4. Western Blotting Analysis of CCN Proteins in Calcified Tissues

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 43-51
    5. Immunohistochemical Analysis of CCN Proteins in Calcified Tissues

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 53-62
    6. Production of Recombinant CCN2 Protein in Escherichia coli

      • Eriko Aoyama, Takako Hattori, Satoshi Kubota, Masaharu Takigawa
      Pages 77-84
    7. Production of Recombinant CCN Proteins by Brevibacillus choshinensis

      • Hiroshi Hanagata, Makoto Mizukami
      Pages 85-93
    8. Production of Recombinant CCN2 Protein by Mammalian Cells

      • Takashi Nishida, Satoshi Kubota, Masaharu Takigawa
      Pages 95-105
    9. In Vitro Transfection with and Expression of CCN Family of Genes

      • Danilo Janune, Masaharu Takigawa
      Pages 107-113
    10. Preparation of Module-Specific Antibodies Against CCN Family Members

      • Satoshi Kubota, Masaharu Takigawa
      Pages 115-126
    11. ELISA of CCN Family Proteins in Body Fluids Including Serum and Plasma

      • Satoshi Kubota, Harumi Kawaki, Masaharu Takigawa
      Pages 127-138
    12. Analysis of Signaling Pathways Activated by CCN Proteins

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 139-143
    13. Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two-Hybrid System

      • Mitsuhiro Hoshijima, Takako Hattori, Masaharu Takigawa
      Pages 145-154
    14. Protocols for Screening Peptide Motifs Binding to CCN Family Proteins

      • Satoshi Kubota, Harumi Kawaki, Masaharu Takigawa
      Pages 155-167
    15. Promoter Analyses of CCN Genes

      • Takanori Eguchi, Satoshi Kubota, Masaharu Takigawa
      Pages 177-185

About this book

 This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstacles with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences and pharmacology of CCN proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, CCN Proteins: Methods and Protocols will be a valuable resource for a wide audience, ranging from the experienced CCN researchers looking for new approaches to junior graduate students just beginning in CCN research.


Editors and Affiliations

  • Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

Bibliographic Information

  • Book Title: CCN Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Masaharu Takigawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6430-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6428-4Published: 13 October 2016

  • Softcover ISBN: 978-1-4939-8197-7Published: 27 June 2018

  • eBook ISBN: 978-1-4939-6430-7Published: 12 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 576

  • Number of Illustrations: 37 b/w illustrations, 67 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access