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Direct PCR-Ribotyping of Clostridium difficile

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Clostridium difficile

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1476))

Abstract

PCR-ribotyping, a method based on heterogeneity of ribosomal intergenic spacer region, is the preferred method for genotyping of Clostridium difficile. Standardly used procedure for PCR-ribotyping is culturing of C. difficile from fecal samples and subsequent typing. In this chapter, we describe a modified PCR-ribotyping method for direct detection of PCR-ribotypes directly in total stool DNA extract, without prior need to isolate C. difficile.

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Author information

Authors and Affiliations

  1. National Laboratory for Health, Environment and Food (NLZOH), Prvomajska 1, 2000, Maribor, Slovenia

    Sandra Janezic

  2. Faculty of Medicine, University of Maribor, Taborska ulica 8, 2000, Maribor, Slovenia

    Sandra Janezic

Authors
  1. Sandra Janezic
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Correspondence to Sandra Janezic .

Editor information

Editors and Affiliations

  1. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    Adam P. Roberts

  2. Department of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, United Kingdom

    Peter Mullany

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Janezic, S. (2016). Direct PCR-Ribotyping of Clostridium difficile . In: Roberts, A., Mullany, P. (eds) Clostridium difficile. Methods in Molecular Biology, vol 1476. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6361-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6361-4_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6359-1

  • Online ISBN: 978-1-4939-6361-4

  • eBook Packages: Springer Protocols

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