Abstract
The development of novel bioorthogonal reactives that can be used to tag biomolecules in vivo has revolutionized the studies of cellular and molecular biology. Among those novel reactive substances, amino acid analogs can be used to label nascent proteins, thus opening new avenues for measuring protein translation rates in vivo with a limited manipulation of the sample. Here, we describe the use of Click-chemistry to tag and separate newly synthesized proteins in mammalian cells that can be used, coupled with western analysis, to estimate the translation rate of any protein of interest.
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Acknowledgment
The authors would like to acknowledge networking support by the Proteostasis COST Action (BM1307).
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Belda-Palazón, B., Ferrando, A., Farràs, R. (2016). Quantitation of Protein Translation Rate In Vivo with Bioorthogonal Click-Chemistry. In: Matthiesen, R. (eds) Proteostasis. Methods in Molecular Biology, vol 1449. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3756-1_24
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DOI: https://doi.org/10.1007/978-1-4939-3756-1_24
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3756-1
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