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A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells

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Auditory and Vestibular Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1427))

Abstract

Nonlinear capacitance (NLC) measures are often used as surrogate measures of outer hair cell (OHC) electromotility (eM), since the two are commonly thought to share many biophysical features. The measurement of NLC is simpler than direct measurements of eM and, therefore, many investigators have adopted it. A standard patch-clamp hardware configuration is sufficient for recording NLC, given the proper software interface. Thus, the approach is cost effective. We use the software jClamp since it is tailored to capacitance measurement. Here we detail steps that we use to measure NLC. The walk through includes isolation of guinea pig OHCs, building voltage commands, recording, and analysis.

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Authors and Affiliations

  1. Department of Surgery, Yale University School of Medicine, 208062, New Haven, CT, 06510, USA

    Lei Song & Joseph Santos-Sacchi

  2. Department of Neurobiology, Yale University School of Medicine, New Haven, CT, 06510, USA

    Joseph Santos-Sacchi

  3. Department of Physiology, Yale University School of Medicine, New Haven, CT, 06510, USA

    Joseph Santos-Sacchi

Authors
  1. Lei Song
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  2. Joseph Santos-Sacchi
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Corresponding author

Correspondence to Joseph Santos-Sacchi .

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Editors and Affiliations

  1. College of Medicine, University of South Florida, Tampa, Florida, USA

    Bernd Sokolowski

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Song, L., Santos-Sacchi, J. (2016). A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology, vol 1427. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3615-1_28

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  • DOI: https://doi.org/10.1007/978-1-4939-3615-1_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3613-7

  • Online ISBN: 978-1-4939-3615-1

  • eBook Packages: Springer Protocols

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