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Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins

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Auditory and Vestibular Research

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1427))

Abstract

Surface plasmon resonance is an optical technique that is utilized for detecting molecular interactions. Binding of a mobile molecule (analyte) to a molecule immobilized on a thin metal film (ligand) changes the refractive index of the film. The angle of extinction of light that is completely reflected after polarized light impinges upon the film, is altered, and monitored as a change in detector position for a dip in reflected intensity (the surface plasmon resonance phenomenon). Because the method strictly detects mass, there is no need to label the interacting components, thus eliminating possible changes of their molecular properties. We have utilized surface plasmon resonance to study interaction of proteins of inner-ear sensory epithelia.

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Acknowledgments

This work was supported by NIH R01 DC000156 and NIH R01 DC004076.

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Authors and Affiliations

  1. Departments of Otolaryngology and Biochemistry-Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI, 48201, USA

    Dennis G. Drescher

  2. Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI, 48201, USA

    Selvakumar Dakshnamurthy, Marian J. Drescher & Neeliyath A. Ramakrishnan

Authors
  1. Dennis G. Drescher
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  2. Selvakumar Dakshnamurthy
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  3. Marian J. Drescher
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  4. Neeliyath A. Ramakrishnan
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Corresponding author

Correspondence to Dennis G. Drescher .

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Editors and Affiliations

  1. College of Medicine, University of South Florida, Tampa, Florida, USA

    Bernd Sokolowski

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Drescher, D.G., Dakshnamurthy, S., Drescher, M.J., Ramakrishnan, N.A. (2016). Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology, vol 1427. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3615-1_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3615-1_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3613-7

  • Online ISBN: 978-1-4939-3615-1

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