Abstract
The caspase-1 enzymatic activity plays a major role in the innate immune response as it regulates the maturation of two major proinflammatory cytokines, the interleukin-1beta (IL-1β) and IL-18. In this chapter, we describe the technique of Western blot to assess caspase-1 activation. This method provides multiple information within one experiment. It allows the detection of both unprocessed and processed caspase-1 and substrates.
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Acknowledgement
We thank Sarah Karabani for proofreading the manuscript. V. Petrilli is supported by the ITMO cancer and the Ligue contre le cancer comité de l’Ain.
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© 2016 Springer Science+Business Media New York
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Guey, B., Petrilli, V. (2016). Assessing Caspase-1 Activation. In: Di Virgilio, F., Pelegrín, P. (eds) NLR Proteins. Methods in Molecular Biology, vol 1417. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3566-6_13
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DOI: https://doi.org/10.1007/978-1-4939-3566-6_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3566-6
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