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The Mitotic Spindle pp 393–401Cite as

Generation and Purification of Tetraploid Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1413))

Abstract

Tetraploid cells are genetically unstable and have the capacity to promote the development and/or progression of human malignancies. It is now estimated that ~40 % of all solid tumors have passed through a tetraploid intermediate stage at some point during their development. Understanding the biological characteristics of tetraploid cells that impart oncogenic properties is therefore a highly relevant and fundamentally important aspect of cancer biology. Here, we describe strategies to efficiently generate and purify tetraploid cells for use in cell biological studies.

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Acknowledgments

N.J.G is a Karin Grunebaum Cancer Research Foundation Fellow in the Shamim and Ashraf Dahod Breast Cancer Research Laboratories and is supported by grants from the Richard and Susan Smith Family Foundation, the Searle Scholars Program, the Melanoma Research Alliance, the Skin Cancer Foundation, the Sarcoma Foundation of America, and the NIH/NCI (K99/R00 CA154531-01).

Author information

Authors and Affiliations

  1. Departments of Pharmacology & Experimental Therapeutics and Medicine, Division of Hematology and Oncology, Boston University School of Medicine, Boston, MA, 02118, USA

    Elizabeth M. Shenk & Neil J. Ganem

Authors
  1. Elizabeth M. Shenk
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  2. Neil J. Ganem
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Corresponding author

Correspondence to Neil J. Ganem .

Editor information

Editors and Affiliations

  1. Massachusetts Institute of Technolo, Cambridge, Massachusetts, USA

    Paul Chang

  2. Dept of Cell & Developmental Biolog, Vanderbilt University, Nashville, Tennessee, USA

    Ryoma Ohi

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© 2016 Springer Science+Business Media New York

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Shenk, E.M., Ganem, N.J. (2016). Generation and Purification of Tetraploid Cells. In: Chang, P., Ohi, R. (eds) The Mitotic Spindle. Methods in Molecular Biology, vol 1413. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3542-0_24

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  • DOI: https://doi.org/10.1007/978-1-4939-3542-0_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3540-6

  • Online ISBN: 978-1-4939-3542-0

  • eBook Packages: Springer Protocols

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