Abstract
Methylation of cytosines plays an important role in epigenetic regulation of gene expression. Several methods exist to determine the methylation status of DNA. Here, we describe a rapid and cost-effective method called Chop-qPCR to determine dynamic changes in the DNA methylation patterns, as they occur for instance in response to environmental stresses.
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Acknowledgements
This work was supported by the Chemical Genomics Centre of the Max Planck Society and its supporting companies AstraZeneca, Bayer CropScience, Bayer HealthCare, Boehringer Ingelheim, and Merck.
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Rausch, S., Laubinger, S. (2016). Rapid Assessment of DNA Methylation Changes in Response to Salicylic Acid by Chop-qPCR. In: Duque, P. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 1398. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3356-3_28
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DOI: https://doi.org/10.1007/978-1-4939-3356-3_28
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3356-3
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