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The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development

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Cancer Chemoprevention

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1379))

Abstract

Epigenetic mechanisms such as DNA methylation, posttranslational modifications of histone proteins, remodeling of nucleosomes, and the expression of noncoding RNAs contribute to the regulation of gene expression for the cell fate determination and tissue development. The disruption of these epigenetic mechanisms, in conjunction with genetic alterations, is a decisive element for cancer development and progression. The cancer phenotype is characterized by global DNA hypomethylation and gene-specific hypermethylation. The methylated DNA immunoprecipitation [MeDIP] is a useful approach currently used to clarify the functional consequences of DNA methylation on cell fate determination and cancer development.

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References

  1. Chen T, Dent SY (2014)‑ Chromatin modifiers and remodellers: regulators of cellular differentiation. Nat Rev Genet 15:93–106

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Jones PA (2012) Functions of DNA methylation: islands, start sites, gene bodies and beyond. Nat Rev Genet 13:484–492

    Article  CAS  PubMed  Google Scholar 

  3. Portela A, Liz J, Nogales V et al (2013) DNA methylation determines nucleosome occupancy in the 5′-CpG islands of tumor suppressor genes. Oncogene 32:5421–5428

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. De Carvalho DD, You JS, Jones PA (2010) DNA methylation and cellular reprogramming. Trends Cell Bio 20:609–617

    Article  Google Scholar 

  5. Brenner C, Fuks F (2006) DNA methyltransferases: facts, clues, mysteries. Curr Top Microbiol Immunol 301:45–66

    CAS  PubMed  Google Scholar 

  6. Chaligne R, Heard E (2014) X-chromosome inactivation in development and cancer. FEBS Lett 588:2514–2522

    Article  CAS  PubMed  Google Scholar 

  7. Jones PA, Baylin SB (2007) The epigenomics of cancer. Cell 128:683–692

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Easwaran H, Tsai HC, Baylin SB (2014) Cancer epigenetics: tumor heterogeneity, plasticity of stem-like states, and drug resistance. Mol Cell 54:716–727

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Taberlay PC, Jones PA (2011) DNA methylation and cancer. Prog Drug Res 67:1–23

    CAS  PubMed  Google Scholar 

  10. Estecio MR, Issa JP (2011) Dissecting DNA hypermethylation in cancer. FEBS Lett 585:2078–2086

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Baylin SB, Jones PA (2011) A decade of exploring the cancer epigenome - biological and translational implications. Nat Rev Cancer 11:726–734

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Figueroa ME, Lugthart S, Li Y et al (2010) DNA methylation signatures identify biologically distinct subtypes in acute myeloid leukemia. Cancer Cell 17:13–27

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Fazi F, Racanicchi S, Zardo G et al (2007) Epigenetic silencing of the myelopoiesis regulator microRNA-223 by the AML1/ETO oncoprotein. Cancer Cell 12:457–466

    Article  CAS  PubMed  Google Scholar 

  14. Fazi F, Zardo G, Gelmetti V et al (2007) Heterochromatic gene repression of the retinoic acid pathway in acute myeloid leukemia. Blood 109:4432–4440

    Article  CAS  PubMed  Google Scholar 

  15. Schoofs T, Muller-Tidow C (2011) DNA methylation as a pathogenic event and as a therapeutic target in AML. Cancer Treat Rev 37(Suppl 1):S13–S18

    Article  CAS  PubMed  Google Scholar 

  16. Gerhauser C (2013) Cancer chemoprevention and nutriepigenetics: state of the art and future challenges. Top Curr Chem 329:73–132

    Article  CAS  PubMed  Google Scholar 

  17. Laird PW (2010) Principles and challenges of genomewide DNA methylation analysis. Nat Rev Genet 11:191–203

    Article  CAS  PubMed  Google Scholar 

  18. Reinders J, Paszkowski J (2010) Bisulfite methylation profiling of large genomes. Epigenomics 2:209–220

    Article  CAS  PubMed  Google Scholar 

  19. Kristensen LS, Hansen LL (2009) PCR-based methods for detecting single-locus DNA methylation biomarkers in cancer diagnostics, prognostics, and response to treatment. Clin Chem 55:1471–1483

    Article  CAS  PubMed  Google Scholar 

  20. Li LC (2007) Designing PCR primer for DNA methylation mapping. Methods Mol Biol 402:371–384

    CAS  PubMed  Google Scholar 

  21. Sorensen AL, Collas P (2009) Immunoprecipitation of methylated DNA. Methods Mol Biol 567:249–262

    Article  PubMed  Google Scholar 

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Acknowledgement

The work was supported by AIRC Start-up grant 4841.

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Authors and Affiliations

  1. Section of Histology & Medical Embryology, Department of Anatomical, Histological, Forensic & Orthopaedic Sciences, Sapienza University of Rome, Via A. Scarpa, 14-16, 00161, Rome, Italy

    Silvia Masciarelli, Teresa Bellissimo, Ilaria Iosue & Francesco Fazi

Authors
  1. Silvia Masciarelli
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  2. Teresa Bellissimo
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  3. Ilaria Iosue
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  4. Francesco Fazi
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Corresponding author

Correspondence to Francesco Fazi .

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Editors and Affiliations

  1. Molecular Cancer Chemoprevention Unit, Molecular Medicine Department; Department of Oncology, Juravinski Cancer Center-McMaster, Regina Elena National Cancer Institute; University Hamilton, Rome; Ontario, Italy

    Sabrina Strano

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Masciarelli, S., Bellissimo, T., Iosue, I., Fazi, F. (2016). The Methylated DNA Immunoprecipitation [MeDIP] to Investigate the Epigenetic Remodeling in Cell Fate Determination and Cancer Development. In: Strano, S. (eds) Cancer Chemoprevention. Methods in Molecular Biology, vol 1379. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3191-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3191-0_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3190-3

  • Online ISBN: 978-1-4939-3191-0

  • eBook Packages: Springer Protocols

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