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Phospho-Proteomics pp 293–306Cite as

Resources for Assignment of Phosphorylation Sites on Peptides and Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1355))

Abstract

Reversible protein phosphorylation is a key regulatory posttranslational modification that plays a significant role in major cellular signaling processes. Phosphorylation events can be systematically identified, quantified, and localized on protein sequence using publicly available bioinformatic tools. Here we present the software tools commonly used by the phosphoproteomics community, discuss their underlying principles of operation, and provide a protocol for large-scale phosphoproteome data analysis using the MaxQuant software suite.

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Acknowledgments

This work was supported by grants from the Chalmers University of Technology (to IM), the Juniorprofessoren-Programm of the Landesstiftung BW, the SFB766 of the Deutsche Forshungsgemeinschaft, and PRIME-XS consortium (to BM).

Author information

Authors and Affiliations

  1. Proteome Center Tuebingen, Interfaculty Institute for Cell Biology, University of Tuebingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany

    Vaishnavi Ravikumar & Boris Macek

  2. Department of Systems and Synthetic Biology, Chalmers University of Technology, Kemivägen 10, 412 96, Göteborg, Sweden

    Ivan Mijakovic

Authors
  1. Vaishnavi Ravikumar
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  2. Boris Macek
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  3. Ivan Mijakovic
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Corresponding authors

Correspondence to Boris Macek or Ivan Mijakovic .

Editor information

Editors and Affiliations

  1. University of Copenhagen, Center for Protein Research, Copenhagen Nm, Denmark

    Louise von Stechow

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Ravikumar, V., Macek, B., Mijakovic, I. (2016). Resources for Assignment of Phosphorylation Sites on Peptides and Proteins. In: von Stechow, L. (eds) Phospho-Proteomics. Methods in Molecular Biology, vol 1355. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-3049-4_20

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  • DOI: https://doi.org/10.1007/978-1-4939-3049-4_20

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-3048-7

  • Online ISBN: 978-1-4939-3049-4

  • eBook Packages: Springer Protocols

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