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Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System

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Baculovirus and Insect Cell Expression Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1350))

Abstract

The production of recombinant proteins using the baculovirus expression vector system (BEVS) in large-scale agitated bioreactors is discussed in this chapter. Detailed methods of the key stages of a batch process, including host cell growth, virus stock amplification and quantification, bioreactor preparation and operation, the infection process, final harvesting, and primary separation steps for recovery of the product are presented. Furthermore, methods involved with advanced on-line monitoring and bioreactor control, which have a significant impact on the overall process success, are briefly discussed.

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Acknowledgements

The authors would like to thank Emma Petiot, Robert Voyer, and Danielle Jacobs for their helpful advice and input throughout the writing of this chapter.

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Ecole Polytechnique de Montreal, 2500 Chemin de Polytechnique, Montreal, Canada

    Christine M. Thompson

  2. National Research Council Canada, Montreal, Canada

    Christine M. Thompson, Johnny Montes & Amine A. Kamen

  3. Department of Chemical Engineering, University of Waterloo, 200 University Ave W., Waterloo, ON, Canada, N2L 3G1

    Marc G. Aucoin

  4. Department of Bioengineering, McGill University, Montreal, Canada

    Amine A. Kamen

Authors
  1. Christine M. Thompson
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  2. Johnny Montes
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  3. Marc G. Aucoin
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  4. Amine A. Kamen
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Corresponding author

Correspondence to Marc G. Aucoin .

Editor information

Editors and Affiliations

  1. Engineering, University of Iowa Dept. Chemical & Biochemical, Iowa City, Iowa, USA

    David W. Murhammer

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Thompson, C.M., Montes, J., Aucoin, M.G., Kamen, A.A. (2016). Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System. In: Murhammer, D. (eds) Baculovirus and Insect Cell Expression Protocols. Methods in Molecular Biology, vol 1350. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3043-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-3043-2_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3042-5

  • Online ISBN: 978-1-4939-3043-2

  • eBook Packages: Springer Protocols

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