Skip to main content
SpringerLink
Log in

Bone Marrow and Fetal Liver Radiation Chimeras

  • Protocol
T-Cell Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1323))

Abstract

Radiation chimeras are prepared by subjecting recipient mice to sublethal or lethal dose of irradiation and injecting them with hematopoietic stem cells (HSC) from untreated donor mice. HSC can be obtained from bone marrow or fetal liver. This technique is a powerful tool when coupled with gene targeting strategies to investigate function of HSCs, thymocyte development, and T cell function. This protocol describes how to produce bone marrow or fetal liver chimeras.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. de la Morena MT, Gatti RA (2011) A history of bone marrow transplantation. Hematol Oncol Clin North Am 25:1–15

    Article  PubMed  Google Scholar 

  2. Hakim F, Fowler DH, Shearer GM, Gress RE (2001) Animal models of acute and chronic graft-versus-host disease. Curr Protoc Immunol Chapter 4:Unit 4 3

    Google Scholar 

  3. Mader SL, Libal NL, Pritchett-Corning K, Yang R, Murphy SJ (2009) Refining timed pregnancies in two strains of genetically engineered mice. Lab Anim (NY) 38:305–310.

    Article  Google Scholar 

  4. Gudmundsson KO, Stull SW, Keller JR (2012) Transplantation of mouse fetal liver cells for analyzing the function of hematopoietic stem and progenitor cells. Methods Mol Biol 879:123–133

    Google Scholar 

Download references

Acknowledgement

The Intramural Research Program of the National Cancer Institute at the National Institutes of Health supports the authors. The authors have no conflicts of interest to disclose.

Author information

Authors and Affiliations

  1. Experimental Transplantation and Immunology Branch, National Cancer Institute, Building 10, CRC, Room 3-3330, Bethesda, MD, 20892, USA

    Francis A. Flomerfelt & Ronald E. Gress

Authors
  1. Francis A. Flomerfelt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ronald E. Gress
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Francis A. Flomerfelt .

Editor information

Editors and Affiliations

  1. Laboratory of Immune Cell Biology, National Institutes of Health National Cancer Institute, Bethesda, Maryland, USA

    Rémy Bosselut

  2. Laboratory of Immune Cell Biology, National Institutes of Health National Cancer Institute, Bethesda, Maryland, USA

    Melanie S. Vacchio

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Flomerfelt, F.A., Gress, R.E. (2016). Bone Marrow and Fetal Liver Radiation Chimeras. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_9

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2809-5_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2808-8

  • Online ISBN: 978-1-4939-2809-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation