Abstract
This chapter reviews the history of the use of the ethanol stability test and surveys the milk compositional factors influencing its result. Developed originally as an indicator of fresh milk quality, it now finds use in some countries as a warning of the lack of suitability for high temperature heat treatment. Relevant factors influencing the heat stability of milk are introduced and mechanisms are proposed and compared for both instability reactions. It is concluded that a fairer option would be to develop a direct rapid heat stability method for use by field operatives.
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References
Ayers SH, Johnson WT Jr (1915) The alcohol test in relation to milk, Bulletin No. 202, US Dept. of Agriculture. Government Printing Office, Washington, DC
Barchiesi-Ferrari CG, Williams-Salinas PA, Salvo-Garrido SI (2007) Milk instability associated with milk composition and seasonal lactation in grazing dairy cows. Pesq Agropec Bras 42:1785–1791
Botaro BG, Real de Lima YV, Aquino AA, Fernandes RHR, Garcia JF, Veiga dos Santos M (2007) Beta-lactoglobulin polymorphism does not affect physico-chemical characteristics and milk stability. Pesq Agropec Bras 42:747–753
Chavez MS, Negri LM, Taverna MA, Cuatrin A (2004) Bovine milk composition parameters affecting the ethanol stability. J Dairy Res 71:201–206
Dahlberg AO, Garner HS (1921) The alcohol test as a means of determining quality of milk for condenseries, Bulletin No. 944, US Dept. of Agriculture. Government Printing Office, Washington, DC
Dalgleish DG, Pouliot Y, Paquin P (1987) Studies on the heat stability of milk. II. Association and dissociation of particles and the effect of added urea. J Dairy Res 54:39–49
Davies DT, White JCD (1958) The relation between the chemical composition of milk and the stability of the casein complex. II. Coagulation by ethanol. J Dairy Res 25:256–266
Donato L, Guyomarc’h F (2009) Formation and properties of whey protein/κ-casein complexes in heated skim milk – a review. Dairy Sci Technol 89:3–29
Donnelly WJ, Horne DS (1986) Relationship between ethanol stability of bovine milk and natural variations in milk composition. J Dairy Res 53:23–33
Fox PF (1981a) Heat-induced changes in milk preceding coagulation. J Dairy Sci 64:2127–2137
Fox PF (1981b) Heat stability of milk: significance of heat-induced acid formation in coagulation. Irish J Food Sci Technol 5:1–11
Fox PF, Hearn CM (1978a) Heat stability of milk: influence of κ-casein hydrolysis. J Dairy Res 45:173–181
Fox PF, Hearn CM (1978b) Heat stability of milk: influence of denaturable proteins and detergents on heat stability. J Dairy Res 45:159–172
Fox PF, Morrissey PA (1977) Reviews of the progress of dairy science: the heat stability of milk. J Dairy Res 44:627–646
Holt C, Muir DD, Ormrod IHL, Zammit VA, Peaker M (1979) Citrate in milk. Ann Report Hannah Res Inst, p 63–69
Horne DS (1984) Steric effects in the coagulation of casein micelles by ethanol. Biopolymers 23:989–993
Horne DS (1986) Steric stabilization and casein micelle stability. J Colloid Interface Sci 111:250–260
Horne DS (1987) Ethanol stability of casein micelles – a hypothesis concerning the role of calcium phosphate. J Dairy Res 54:389–395
Horne DS (1998) Casein interactions: casting light on the black boxes, the structure of dairy products. Int Dairy J 8:171–177
Horne DS (2003) Ethanol stability. In: Fox PF, McSweeney PLH (eds) Advanced dairy chemistry, vol 1, 3rd edn, Proteins. Kluwer Academic/Plenum Publishers, New York, pp 974–999
Horne DS (2006) Casein micelle structure: model and muddles. Curr Opin Colloid Interface Sci 11:148–153
Horne DS, Davidson CM (1986) The effect of environmental conditions on the steric stabilization of casein micelles. Colloid Polym Sci 264:727–734
Horne DS, Davidson CM (1987) Alcohol stability of milk. Anomalous effects with trifluoroethanol. Milchwissenschaft 42:509–512
Horne DS, Muir DD (1990) Alcohol and heat stability of milk protein. J Dairy Sci 73:3613–3626
Horne DS, Parker TG (1980) The pH sensitivity of the ethanol stability of individual cow milks. Neth Milk Dairy J 34:126–130
Horne DS, Parker TG (1981a) Factors affecting the ethanol stability of bovine milk. I. Effect of serum phase components. J Dairy Res 48:273–284
Horne DS, Parker TG (1981b) Factors affecting the ethanol stability of bovine milk. II. The origin of the pH transition. J Dairy Res 48:285–91
Horne DS, Parker TG (1981c) Factors affecting the ethanol stability of bovine casein micelles. III. Substitution of ethanol by other organic solvents. Int J Biol Macromol 3:399–402
Horne DS, Parker TG (1982) Factors affecting the ethanol stability of bovine milk. V. Effects of chemical modification of milk protein. J Dairy Res 49:449–457
Horne DS, Parker TG, Donnelly WJ, Davies DT (1986) Factors affecting the ethanol stability of bovine milk. VII. Lactational and compositional effects. J Dairy Res 53:407–417
Huppertz T, DeKruif CG (2007) Ethanol stability of casein micelles cross-linked with transglutaminase. Int Dairy J 17:436–441
Jenness R, Koops J (1962) Preparation and properties of a salt solution which simulates milk ultrafiltrate. Neth Milk Dairy J 16:153–164
Manson W, Carolan T (1980) Formation of lysinoalanine from individual bovine caseins. J Dairy Res 47:193–198
Manson W, Carolan T, Annan WD (1985) Products of heat-promoted reaction between urea and the protein fraction of milk. J Dairy Res 52:401–407
Marques LT, Zanela MB, Ribeiro MER, Stumpf W Jr, Fischer V (2007) Occurrence of unstable to alcohol 76% and non-acid milk (LINA) and influence on physicochemical aspects of milk. R Bras Agrociencia 13:91–97
Metwalli AAM, Van Boekel MAJS (1996) Effect of urea on heat coagulation of milk. Neth Milk Dairy J 50:459–476
Metwalli AAM, Metwalli NH, Van Boekel MAJS (1996) Effect of urea on heat-induced changes in milk. Neth Milk Dairy J 50:427–457
Mezdour S, Brulé G, Korolczuk J (2006) Physicochemical analysis of casein solubility in water ethanol solutions. Lait 86:435–452
Miller PG, Sommer HH (1940) The coagulation temperature of milk as affected by pH, ionic salts, evaporation and previous heat treatment. J Dairy Sci 23:405–421
Mitamura K (1937) Studies on the alcohol coagulation of fresh cow milk. J Fac Agric Hokkaido Univ 41:97–362
Morrissey PA (1969) The heat stability of milk as affected by variation in pH and milk salts. J Dairy Res 36:343–351
Muir DD, Sweetsur AWM (1977) Effect of urea on the heat coagulation of the caseinate complex in skim-milk. J Dairy Res 44:249–257
O’Connell JE, Fox PF (2000) The two-stage coagulation of milk proteins in the minimum of the heat coagulation time- pH profile of milk: effect of casein micelle size. J Dairy Sci 83:378–386
O’Connell JE, Fox PF (2003) Heat-induced coagulation of milk. In: Fox PF, McSweeney PLH (eds) Advanced dairy chemistry, vol 1, 3rd edn, Proteins. Kluwer Academic/Plenum Publishers, New York, pp 879–945
O’Connell JE, Kelly A, Auty MAE, Fox PF, DeKruif CG (2001a) Ethanol-dependent heat-induced dissociation of casein micelles. J Agric Food Chem 49:4420–4423
O’Connell JE, Kelly A, Fox PF, DeKruif CG (2001b) Mechanism for ethanol-dependent heat-induced dissociation of casein micelles. J Agric Food Chem 49:4424–4428
Parker TG, Horne DS, Dalgleish DG (1979) Theory for the heat-induced coagulation of a Type A milk. J Dairy Res 46:377–380
Pierre A (1985) Milk coagulation by alcohol. Studies on the solubility of milk calcium and phosphate in alcoholic solutions. Lait 65:201–212
Ramsdell GA, Johnson WT Jr, Evans FR (1931) A test for the detection of milk unstable to heat. J Dairy Sci 14:93–106
Rose D (1961a) Variations in the heat stability and composition of milk from individual cows during lactation. J Dairy Sci 44:430–441
Rose D (1961b) Factors affecting the pH-sensitivity of the heat stability of milk from individual cows. J Dairy Sci 44:1405–1413
Rose D (1962) Factors affecting the heat stability of milk. J Dairy Sci 45:1305–1311
Rose D (1963) Heat stability of bovine milk. A review. Dairy Sci Abstracts 25:45–52
Shekarforoush SS, Zareie M, Mohebbi Fani M (2003) Efficacy of alcohol coagulation test for evaluation of bulk milk quality. J Fac Vet Med Univ Teh 58:169–175
Singh H, Creamer LK (1992) Heat stability of milk. In: Fox PF (ed) Advanced dairy chemistry, vol 1, 2nd edn, Proteins. Elsevier Applied Science, London, pp 621–656
Sweetsur AWM, White JCD (1974) Studies on the heat stability of milk protein. I. Interconversion of Type A and Type B milk heat stability curves. J Dairy Res 41:349–358
Tessier H, Rose D (1964) Influence of kappa casein and beta lactoglobulin on the heat stability of skimmilk. J Dairy Sci 47:1047–1051
Tsioulpas A, Grandison AS, Lewis MJ (2007) Changes in physical properties of bovine milk from the colostrum period to early lactation. J Dairy Sci 90:5012–5017
Walstra P, Bloomfield VA, Wei G, Jenness R (1981) Effects of chymosin action on hydrodynamic diameter of casein micelles. Biochim Biophys Acta 669:258–259
White JCD, Davies DT (1958) The relation between the chemical composition of milk and stability of the caseinate complex. I. General introduction, description of samples, methods and chemical composition of samples. J Dairy Res 30:171–189
White JCD, Davies DT (1966) The stability of milk protein to heat. III. Objective measurement of heat stability of milk. J Dairy Res 36:93–102
Zanela MB, Fischer V, Rocha Ribiero ME, Barbosa RS, Marques LT, Stumpf W Jr, Zanela C (2006) Unstable non-acid milk and milk composition of Jersey cows on feed restriction. Pesq Agropec Bras 41:835–840
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Horne, D.S. (2016). Ethanol Stability and Milk Composition. In: McSweeney, P., O'Mahony, J. (eds) Advanced Dairy Chemistry. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2800-2_9
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DOI: https://doi.org/10.1007/978-1-4939-2800-2_9
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