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The Use of Yeast Surface Display in Biofuel Cells

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Yeast Surface Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1319))

Abstract

Biofuel cells are electrochemical devices which convert chemical energy to electricity using biochemical pathways and redox enzymes. In enzymatic fuel cells purified redox enzymes catalyze the reactions in the anode and cathode compartments whereas in microbial fuel cells (MFCs) the entire metabolism of the microorganisms is exploited. Here, a hybrid biofuel cell concept is presented, which is based on yeast surface display (YSD) of redox enzymes to catalyze the different cell reactions.

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References

  1. Bullen RA, Arnot TC, Lakeman JB et al (2006) Biofuel cells and their development. Biosens Bioelectron 22:2015–2045

    Article  Google Scholar 

  2. Logan BE, Regan JM (2006) Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol 14:512–518

    Article  CAS  PubMed  Google Scholar 

  3. Lovley DR (2006) Bug juice: harvesting electricity with microorganisms. Nat Rev Microbiol 4:497–508

    Article  CAS  PubMed  Google Scholar 

  4. Cooney MJ, Svoboda V, Lau C et al (2008) Enzyme catalysed biofuel cells. Energy Environ Sci 1:320

    Article  CAS  Google Scholar 

  5. Cracknell JA, Vincent KA, Armstrong FA (2008) Enzymes as working or inspirational electrocatalysts for fuel cells and electrolysis. Chem Rev 108:2439–2461

    Article  CAS  PubMed  Google Scholar 

  6. Lovley DR (2006) Microbial fuel cells: novel microbial physiologies and engineering approaches. Curr Opin Biotechnol 17:327–332

    Article  CAS  PubMed  Google Scholar 

  7. Logan BE, Hamelers B, Rozendal R et al (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192

    Article  CAS  PubMed  Google Scholar 

  8. Logan BE, Regan JM (2006) Microbial fuel cells—challenges and applications. Environ Sci Technol 40:5172–5180

    Article  CAS  PubMed  Google Scholar 

  9. Fishilevich S, Amir L, Fridman Y et al (2009) Surface display of redox enzymes in microbial fuel cells. J Am Chem Soc 131:12052–12053

    Article  CAS  PubMed  Google Scholar 

  10. Szczupak A, Kol-Kalman D, Alfonta L (2012) A hybrid biocathode: surface display of O2-reducing enzymes for microbial fuel cell applications. Chem Commun 48:49–51

    Article  CAS  Google Scholar 

  11. Bahartan K, Amir L, Israel A et al (2012) In Situ fuel processing in a microbial fuel cell. ChemSusChem 5:1820–1825

    Article  CAS  PubMed  Google Scholar 

  12. Bahartan K, Gun J, Sladkevich S et al (2012) Encapsulation of yeast displaying glucose oxidase on their surface in graphene oxide hydrogel scaffolding and its bioactivation. Chem Commun 48:11957–11959

    Article  CAS  Google Scholar 

  13. Colby DW, Kellogg BA, Graff CP et al (2004) Engineering antibody affinity by yeast surface display. Methods Enzymol 388:348–358

    CAS  PubMed  Google Scholar 

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Acknowledgment

Israel Science Foundation (I.S.F.) is acknowledged for financial support of the research mentioned here (grants numbers: 763/07, 1693/07 and 232/13, L.A.) as well as ERC-Stg project: ProtLEGO # 260647 (L.A.). A.S. is grateful to the Azrieli Foundation for the award of an Azrieli Fellowship as well as the interdisciplinary Nanotechnology Merage program for a Ph.D. fellowship.

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Correspondence to Lital Alfonta .

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© 2015 Springer Science+Business Media New York

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Szczupak, A., Alfonta, L. (2015). The Use of Yeast Surface Display in Biofuel Cells. In: Liu, B. (eds) Yeast Surface Display. Methods in Molecular Biology, vol 1319. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2748-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-2748-7_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2747-0

  • Online ISBN: 978-1-4939-2748-7

  • eBook Packages: Springer Protocols

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