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Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins

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NF-kappa B

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1280))

Abstract

Among NF-κB transcription factors, c-Rel and c-Rel-derived proteins, including v-Rel, are the only ones that have shown consistent and frank transforming activity in cell culture. In particular, viral, chicken, mouse, and human Rel proteins can rapidly transform primary chicken spleen and bone marrow cells. Overexpression of a human Rel protein missing a C-terminal transactivation domain can also enhance the transformed state of the human B-lymphoma cell line BJAB. As described in this chapter, these in vitro assays can be used to quantitatively assess the transforming activity of Rel proteins.

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Acknowledgments

Research in the authors’ laboratories on transformation of cells by Rel proteins was supported by NIH grants CA047763 (T.D.G.) and CA054999 (C.G.).

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Authors and Affiliations

  1. Department of Biology, Boston University, 5 Cummington Mall, Boston, MA, 02215, USA

    Thomas D. Gilmore

  2. Center for Advanced Biotechnology and Medicine, RBHS-Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    Céline Gélinas

  3. Department of Biochemistry & Molecular Biology, RBHS-Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA

    Céline Gélinas

  4. Rutgers Cancer Institute of New Jersey, New Brunswick, NJ, 08903, USA

    Céline Gélinas

Authors
  1. Thomas D. Gilmore
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  2. Céline Gélinas
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Correspondence to Thomas D. Gilmore .

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Editors and Affiliations

  1. Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA

    Michael J. May

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Gilmore, T.D., Gélinas, C. (2015). Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins. In: May, M. (eds) NF-kappa B. Methods in Molecular Biology, vol 1280. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2422-6_26

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  • DOI: https://doi.org/10.1007/978-1-4939-2422-6_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2421-9

  • Online ISBN: 978-1-4939-2422-6

  • eBook Packages: Springer Protocols

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