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In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans

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Mitochondrial Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1265))

Abstract

Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.

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Author information

Authors and Affiliations

  1. Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences (SILS), Faculty of Science (FNWI), University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands

    Reuben L. Smith, Richard de Boer & Hans van der Spek

  2. Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, Antwerp University, Groenenborgerlaan 171, Antwerp, 2020, Belgium

    Winnok H. De Vos

  3. Cell Systems and Imaging Research Group, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, Ghent, 9000, Belgium

    Winnok H. De Vos

  4. van Leeuwenhoek Center for Advanced Microscopy, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands

    Erik M. M. Manders

Authors
  1. Reuben L. Smith
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  2. Winnok H. De Vos
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  3. Richard de Boer
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  4. Erik M. M. Manders
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  5. Hans van der Spek
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Corresponding author

Correspondence to Hans van der Spek .

Editor information

Editors and Affiliations

  1. Midwestern University, Glendale, Arizona, USA

    Volkmar Weissig

  2. ISANH, Paris, France

    Marvin Edeas

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Smith, R.L., De Vos, W.H., de Boer, R., Manders, E.M.M., van der Spek, H. (2015). In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans . In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine. Methods in Molecular Biology, vol 1265. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2288-8_26

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  • DOI: https://doi.org/10.1007/978-1-4939-2288-8_26

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2287-1

  • Online ISBN: 978-1-4939-2288-8

  • eBook Packages: Springer Protocols

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